Antimicrobial effects of acidic hot-spring water on Staphylococcus aureus strains isolated from atopic dermatitis patients

Hisanori Akiyama, Osamu Yamasaki, Joji Tada, Kazuo Kubota, Jirô Arata

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The present study examined the antimicrobial effects of acidic hot-spring water on Staphylococcus aureus strains isolated from atopic dermatitis (AD) patients. Plasma coagulation by S. aureus cells was not detected in plasma containing acidic hot-spring water (60%, pH 5.4) or hydrochloric acid (pH 5.0) after incubation for 24 h. S. aureus cells did not grow in Mueller-Hinton broth with acidic hot-spring water (50%, pH 4.4) after 24 h incubation. The colony counts of S. aureus cells in tryptic soy broth containing acidic hot-spring water (60%, pH 3.9) were over ten times lower than those in tryptic soy broth alone after incubation for 24 h (P <0.01). A membranous structure (an immature biofilm) was formed on the coverslips of tissue culture dishes by S. aureus cells in plasma after incubation for 24 h, although the colony counts of S. aureus cells in the immature biofilms in plasma containing acidic hot-spring water (60%, pH 5.4) were about eight times lower than those in plasma alone after incubation for 24 h (P <0.01). The colony counts of S. aureus cells that attached on coverslips in plasma containing acidic hot-spring water (60%, pH 5.4) or hydrochloric acid (pH 5.4) were over 1000 times lower than those in plasma alone after incubation for 24 h. These results suggest that 50% acidic hot-spring water has a bacteriostatic effect, 60% acidic hot-spring water has a moderate bactericidal effect against floating S. aureus cells and those cells in a biofilm, and, 60% acidic hot-spring water has an inhibitory effect on plasma coagulation and attachment of S. aureus cells. Furthermore, our present results suggest that a small amount of some ions in hot-spring water such as manganese and iodide ions are very important for a bactericidal activity of hot-spring water as well as the low pH condition. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)112-118
Number of pages7
JournalJournal of Dermatological Science
Volume24
Issue number2
DOIs
Publication statusPublished - 2000

Fingerprint

Hot springs
Hot Springs
Atopic Dermatitis
Staphylococcus aureus
Water
Plasmas
Biofilms
Hydrochloric Acid
Coagulation
Ions
Tissue culture
Iodides
Manganese
Plasma Cells

Keywords

  • Acidic hot-spring water
  • Atopic dermatitis
  • Attachment
  • Plasma coagulation
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Dermatology

Cite this

Antimicrobial effects of acidic hot-spring water on Staphylococcus aureus strains isolated from atopic dermatitis patients. / Akiyama, Hisanori; Yamasaki, Osamu; Tada, Joji; Kubota, Kazuo; Arata, Jirô.

In: Journal of Dermatological Science, Vol. 24, No. 2, 2000, p. 112-118.

Research output: Contribution to journalArticle

@article{afedf0653c96406394b4cd8e18b0595b,
title = "Antimicrobial effects of acidic hot-spring water on Staphylococcus aureus strains isolated from atopic dermatitis patients",
abstract = "The present study examined the antimicrobial effects of acidic hot-spring water on Staphylococcus aureus strains isolated from atopic dermatitis (AD) patients. Plasma coagulation by S. aureus cells was not detected in plasma containing acidic hot-spring water (60{\%}, pH 5.4) or hydrochloric acid (pH 5.0) after incubation for 24 h. S. aureus cells did not grow in Mueller-Hinton broth with acidic hot-spring water (50{\%}, pH 4.4) after 24 h incubation. The colony counts of S. aureus cells in tryptic soy broth containing acidic hot-spring water (60{\%}, pH 3.9) were over ten times lower than those in tryptic soy broth alone after incubation for 24 h (P <0.01). A membranous structure (an immature biofilm) was formed on the coverslips of tissue culture dishes by S. aureus cells in plasma after incubation for 24 h, although the colony counts of S. aureus cells in the immature biofilms in plasma containing acidic hot-spring water (60{\%}, pH 5.4) were about eight times lower than those in plasma alone after incubation for 24 h (P <0.01). The colony counts of S. aureus cells that attached on coverslips in plasma containing acidic hot-spring water (60{\%}, pH 5.4) or hydrochloric acid (pH 5.4) were over 1000 times lower than those in plasma alone after incubation for 24 h. These results suggest that 50{\%} acidic hot-spring water has a bacteriostatic effect, 60{\%} acidic hot-spring water has a moderate bactericidal effect against floating S. aureus cells and those cells in a biofilm, and, 60{\%} acidic hot-spring water has an inhibitory effect on plasma coagulation and attachment of S. aureus cells. Furthermore, our present results suggest that a small amount of some ions in hot-spring water such as manganese and iodide ions are very important for a bactericidal activity of hot-spring water as well as the low pH condition. (C) 2000 Elsevier Science Ireland Ltd.",
keywords = "Acidic hot-spring water, Atopic dermatitis, Attachment, Plasma coagulation, Staphylococcus aureus",
author = "Hisanori Akiyama and Osamu Yamasaki and Joji Tada and Kazuo Kubota and Jir{\^o} Arata",
year = "2000",
doi = "10.1016/S0923-1811(00)00091-8",
language = "English",
volume = "24",
pages = "112--118",
journal = "Journal of Dermatological Science",
issn = "0923-1811",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Antimicrobial effects of acidic hot-spring water on Staphylococcus aureus strains isolated from atopic dermatitis patients

AU - Akiyama, Hisanori

AU - Yamasaki, Osamu

AU - Tada, Joji

AU - Kubota, Kazuo

AU - Arata, Jirô

PY - 2000

Y1 - 2000

N2 - The present study examined the antimicrobial effects of acidic hot-spring water on Staphylococcus aureus strains isolated from atopic dermatitis (AD) patients. Plasma coagulation by S. aureus cells was not detected in plasma containing acidic hot-spring water (60%, pH 5.4) or hydrochloric acid (pH 5.0) after incubation for 24 h. S. aureus cells did not grow in Mueller-Hinton broth with acidic hot-spring water (50%, pH 4.4) after 24 h incubation. The colony counts of S. aureus cells in tryptic soy broth containing acidic hot-spring water (60%, pH 3.9) were over ten times lower than those in tryptic soy broth alone after incubation for 24 h (P <0.01). A membranous structure (an immature biofilm) was formed on the coverslips of tissue culture dishes by S. aureus cells in plasma after incubation for 24 h, although the colony counts of S. aureus cells in the immature biofilms in plasma containing acidic hot-spring water (60%, pH 5.4) were about eight times lower than those in plasma alone after incubation for 24 h (P <0.01). The colony counts of S. aureus cells that attached on coverslips in plasma containing acidic hot-spring water (60%, pH 5.4) or hydrochloric acid (pH 5.4) were over 1000 times lower than those in plasma alone after incubation for 24 h. These results suggest that 50% acidic hot-spring water has a bacteriostatic effect, 60% acidic hot-spring water has a moderate bactericidal effect against floating S. aureus cells and those cells in a biofilm, and, 60% acidic hot-spring water has an inhibitory effect on plasma coagulation and attachment of S. aureus cells. Furthermore, our present results suggest that a small amount of some ions in hot-spring water such as manganese and iodide ions are very important for a bactericidal activity of hot-spring water as well as the low pH condition. (C) 2000 Elsevier Science Ireland Ltd.

AB - The present study examined the antimicrobial effects of acidic hot-spring water on Staphylococcus aureus strains isolated from atopic dermatitis (AD) patients. Plasma coagulation by S. aureus cells was not detected in plasma containing acidic hot-spring water (60%, pH 5.4) or hydrochloric acid (pH 5.0) after incubation for 24 h. S. aureus cells did not grow in Mueller-Hinton broth with acidic hot-spring water (50%, pH 4.4) after 24 h incubation. The colony counts of S. aureus cells in tryptic soy broth containing acidic hot-spring water (60%, pH 3.9) were over ten times lower than those in tryptic soy broth alone after incubation for 24 h (P <0.01). A membranous structure (an immature biofilm) was formed on the coverslips of tissue culture dishes by S. aureus cells in plasma after incubation for 24 h, although the colony counts of S. aureus cells in the immature biofilms in plasma containing acidic hot-spring water (60%, pH 5.4) were about eight times lower than those in plasma alone after incubation for 24 h (P <0.01). The colony counts of S. aureus cells that attached on coverslips in plasma containing acidic hot-spring water (60%, pH 5.4) or hydrochloric acid (pH 5.4) were over 1000 times lower than those in plasma alone after incubation for 24 h. These results suggest that 50% acidic hot-spring water has a bacteriostatic effect, 60% acidic hot-spring water has a moderate bactericidal effect against floating S. aureus cells and those cells in a biofilm, and, 60% acidic hot-spring water has an inhibitory effect on plasma coagulation and attachment of S. aureus cells. Furthermore, our present results suggest that a small amount of some ions in hot-spring water such as manganese and iodide ions are very important for a bactericidal activity of hot-spring water as well as the low pH condition. (C) 2000 Elsevier Science Ireland Ltd.

KW - Acidic hot-spring water

KW - Atopic dermatitis

KW - Attachment

KW - Plasma coagulation

KW - Staphylococcus aureus

UR - http://www.scopus.com/inward/record.url?scp=0033734721&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033734721&partnerID=8YFLogxK

U2 - 10.1016/S0923-1811(00)00091-8

DO - 10.1016/S0923-1811(00)00091-8

M3 - Article

C2 - 11064246

AN - SCOPUS:0033734721

VL - 24

SP - 112

EP - 118

JO - Journal of Dermatological Science

JF - Journal of Dermatological Science

SN - 0923-1811

IS - 2

ER -