Antimicrobial and antibiofilm effects of abietic acid on cariogenic Streptococcus mutans

Research output: Contribution to journalArticle

Abstract

Dental caries is a type of oral microbiome dysbiosis and biofilm infection that affects oral and systemic conditions. For healthy life expectancy, natural bacteriostatic products are ideal for daily and lifetime use as anti-oral infection agents. This study aimed to evaluate the inhibitory effects of abietic acid, a diterpene derived from pine rosin, on the in vitro growth of cariogenic bacterial species, Streptococcus mutans. The effective minimum inhibitory concentration of abietic acid was determined through observation of S. mutans growth, acidification, and biofilm formation. The inhibitory effects of abietic acid on the bacterial membrane were investigated through the use of in situ viability analysis and scanning electron microscopic analysis. Cytotoxicity of abietic acid was also examined in the context of several human cell lines using tetrazolium reduction assay. Abietic acid was found to inhibit key bacterial growth hallmarks such as colony forming ability, adenosine triphosphate activity (both planktonic and biofilm), acid production, and biofilm formation. Abietic acid was identified as bacteriostatic, and this compound caused minimal damage to the bacterial membrane. This action was different from that of povidone-iodine or cetylpyridinium chloride. Additionally, abietic acid was significantly less cytotoxic compared to povidone-iodine, and it exerted lower toxicity towards epithelial cells and fibroblasts compared to that against monocytic cells. These data suggest that abietic acid may prove useful as an antibacterial and antibiofilm agent for controlling S. mutans infection.

Original languageEnglish
JournalOdontology
DOIs
Publication statusAccepted/In press - Jan 1 2019

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Streptococcus mutans
Biofilms
Povidone-Iodine
Growth
Infection
Dysbiosis
Cetylpyridinium
Membranes
abietic acid
Diterpenes
Microbiota
Dental Caries
Microbial Sensitivity Tests
Life Expectancy
Fibroblasts
Adenosine Triphosphate
Epithelial Cells
Observation
Electrons
Anti-Bacterial Agents

Keywords

  • Abietic acid
  • Antimicrobial
  • Oral biofilm
  • Streptococcus mutans
  • Terpene

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

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title = "Antimicrobial and antibiofilm effects of abietic acid on cariogenic Streptococcus mutans",
abstract = "Dental caries is a type of oral microbiome dysbiosis and biofilm infection that affects oral and systemic conditions. For healthy life expectancy, natural bacteriostatic products are ideal for daily and lifetime use as anti-oral infection agents. This study aimed to evaluate the inhibitory effects of abietic acid, a diterpene derived from pine rosin, on the in vitro growth of cariogenic bacterial species, Streptococcus mutans. The effective minimum inhibitory concentration of abietic acid was determined through observation of S. mutans growth, acidification, and biofilm formation. The inhibitory effects of abietic acid on the bacterial membrane were investigated through the use of in situ viability analysis and scanning electron microscopic analysis. Cytotoxicity of abietic acid was also examined in the context of several human cell lines using tetrazolium reduction assay. Abietic acid was found to inhibit key bacterial growth hallmarks such as colony forming ability, adenosine triphosphate activity (both planktonic and biofilm), acid production, and biofilm formation. Abietic acid was identified as bacteriostatic, and this compound caused minimal damage to the bacterial membrane. This action was different from that of povidone-iodine or cetylpyridinium chloride. Additionally, abietic acid was significantly less cytotoxic compared to povidone-iodine, and it exerted lower toxicity towards epithelial cells and fibroblasts compared to that against monocytic cells. These data suggest that abietic acid may prove useful as an antibacterial and antibiofilm agent for controlling S. mutans infection.",
keywords = "Abietic acid, Antimicrobial, Oral biofilm, Streptococcus mutans, Terpene",
author = "Yuki Ito and Takashi Ito and Keisuke Yamashiro and Fumi Mineshiba and Kimito Hirai and Kazuhiro Omori and Tadashi Yamamoto and Shogo Takashiba",
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AU - Ito, Yuki

AU - Ito, Takashi

AU - Yamashiro, Keisuke

AU - Mineshiba, Fumi

AU - Hirai, Kimito

AU - Omori, Kazuhiro

AU - Yamamoto, Tadashi

AU - Takashiba, Shogo

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