Anticardiolipin antibodies recognize β2-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface

Eiji Matsuura, Yoshiko Igarashi, Tatsuji Yasuda, Douglas A. Triplett, Takao Koike

Research output: Contribution to journalArticle

543 Citations (Scopus)

Abstract

Anticardiolipin antibodies (aCL) derived from the sera of individuals exhibiting the antiphospholipid syndrome (APS) directly bind to β2- glycoprotein I (β2-GPI), which is adsorbed to an oxidized polystyrene surface. Oxygen atoms were introduced on a polystyrene surface by irradiation with electron or γ-ray radiation. X-ray photoelectron spectroscopy revealed the irradiated surfaces were oxidized to generate C-O and C=O moieties. aCL derived from either APS patients or (NZW x BXSB)F1 mice bound to β2-GPI coated on the irradiated plates, depending on the radiation dose. Antibody binding to β2-GPI on the irradiated plates was competitively inhibited by simultaneous addition of cardiolipin (CL)-coated latex beads mixed together with β2-GPI but were unaffected by addition of excess β2-GPI, CL micelles, or CL-coated latex beads alone. There was a high correlation between binding values of aCL in sera from 40 APS patients obtained by the anti-β2-GPI enzyme-linked immunosorbent assay (ELISA) using the irradiated plates and those by the β2-GPI-dependent aCL ELISA. Therefore, aCL have specificity for an epitope on β2-GPI. This epitope is expressed by a conformational change occurring when β2-GPI interacts with an oxygen- substituted solid phase surface.

Original languageEnglish
Pages (from-to)457-462
Number of pages6
JournalJournal of Experimental Medicine
Volume179
Issue number2
Publication statusPublished - Feb 1 1994

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Anticardiolipin Antibodies
Glycoproteins
Oxygen
Cardiolipins
Antiphospholipid Syndrome
Polystyrenes
Microspheres
Epitopes
Enzyme-Linked Immunosorbent Assay
Radiation
Photoelectron Spectroscopy
Antibody Specificity
Micelles
Serum
Electrons
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Anticardiolipin antibodies recognize β2-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface. / Matsuura, Eiji; Igarashi, Yoshiko; Yasuda, Tatsuji; Triplett, Douglas A.; Koike, Takao.

In: Journal of Experimental Medicine, Vol. 179, No. 2, 01.02.1994, p. 457-462.

Research output: Contribution to journalArticle

Matsuura, Eiji ; Igarashi, Yoshiko ; Yasuda, Tatsuji ; Triplett, Douglas A. ; Koike, Takao. / Anticardiolipin antibodies recognize β2-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface. In: Journal of Experimental Medicine. 1994 ; Vol. 179, No. 2. pp. 457-462.
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