Anticarcinogenic antioxidants as inhibitors against intracellular oxidative stress

Qing Feng, Takeshi Kumagai, Yasuyoshi Torii, Yoshimasa Nakamura, Toshihiko Osawa, Koji Uchida

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Oxidative stress has been implicated in the pathogenesis of numerous diseases, including cancer. In the present study, the protective effect of natural anti-oxidants, such as quercetin and tea polyphenols, on intracellular oxidative stress was studied. Here we report a novel function of quercetin and tea polyphenols, as potential inhibitors of 4-hydroxy-2-nonenal (HNE)-induced intracellular oxidative stress and cytotoxicity. In rat liver epithelial RL34 cells, a potent electrophile HNE dramatically induced the productions of reactive oxygen species (ROS), which correlated well with the reduction in cell viability. We found that quercetin and tea polyphenols, such as epigallocatechin gallate and theaflavins and their gallate esters, significantly inhibited the HNE-induced ROS production and cytotoxicity. In addition, HNE induced a transient decrease in the mitochondrial membrane potential (ΔΨ), which was also retarded by the antioxidants. These data suggest that the antioxidants, such as quercetin and tea polyphenols, are inhibitors against mitochondrial ROS production. Abbreviations: EGCG, epigallocatechin gallate; HNE, 4-hydroxy-2-nonenal; ROS, reactive oxygen species; DCFH-DA, 2′,7′-dichlorofluorescin diacetate; DiOC6(3), 3,3′-dihexyloxacarbocyanine iodide; GSH, glutathione; TPA, 12-O-tetradecanoylphorbol-13-acetate.

Original languageEnglish
Pages (from-to)779-788
Number of pages10
JournalFree Radical Research
Volume35
Issue number6
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Oxidative stress
Oxidative Stress
Antioxidants
Quercetin
Polyphenols
Tea
Reactive Oxygen Species
Cytotoxicity
Mitochondrial Membrane Potential
Iodides
Tetradecanoylphorbol Acetate
Oxidants
Liver
Glutathione
4-hydroxy-2-nonenal
Rats
Cell Survival
Esters
Acetates
Epithelial Cells

Keywords

  • 4-hydroxy-2-nonenal
  • Antioxidant
  • Mitochondria
  • Oxidative stress
  • Quercetin
  • Tea polyphenols

ASJC Scopus subject areas

  • Biochemistry

Cite this

Feng, Q., Kumagai, T., Torii, Y., Nakamura, Y., Osawa, T., & Uchida, K. (2001). Anticarcinogenic antioxidants as inhibitors against intracellular oxidative stress. Free Radical Research, 35(6), 779-788.

Anticarcinogenic antioxidants as inhibitors against intracellular oxidative stress. / Feng, Qing; Kumagai, Takeshi; Torii, Yasuyoshi; Nakamura, Yoshimasa; Osawa, Toshihiko; Uchida, Koji.

In: Free Radical Research, Vol. 35, No. 6, 2001, p. 779-788.

Research output: Contribution to journalArticle

Feng, Q, Kumagai, T, Torii, Y, Nakamura, Y, Osawa, T & Uchida, K 2001, 'Anticarcinogenic antioxidants as inhibitors against intracellular oxidative stress', Free Radical Research, vol. 35, no. 6, pp. 779-788.
Feng, Qing ; Kumagai, Takeshi ; Torii, Yasuyoshi ; Nakamura, Yoshimasa ; Osawa, Toshihiko ; Uchida, Koji. / Anticarcinogenic antioxidants as inhibitors against intracellular oxidative stress. In: Free Radical Research. 2001 ; Vol. 35, No. 6. pp. 779-788.
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