Antibody to heat shock protein can be used for early serological monitoring of Helicobacter pylori eradication treatment

Naoko Yunoki, Kenji Yokota, Motowo Mizuno, Yoshiro Kawahara, Masayasu Adachi, Hiroyuki Okada, Shyunji Hayashi, Yoshikazu Hirai, Keiji Oguma, Takao Tsuji

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Infection with Helicobacter pylori induces humoral immune responses against various antigens of the bacterium. Heat shock proteins (hsps) are immunodominant antigens in various diseases including H. pylori infection. In the present study, we measured the anti-hsp antibody titers in 42 patients with H. pylori-infected peptic ulcers during a bacterial eradication study. The patients were treated with a proton pump inhibitor and antimicrobial agents to eradicate the organism. Their sera were obtained at pretreatment and at 1 month and 6 months after the eradication therapy. The titers of immunoglobulin G antibodies to the H. pylori hsp, whole-cell lysate, and urease (30-kDa subunit) antigens in serum were measured by a capture enzyme- linked immunosorbent assay. The levels of H. pylori hsp60 antibodies in sera collected 1 month after treatment had declined significantly, even when changes in the titers of antibodies to whole-cell and urease antigens were not apparent. These results suggest that measurement of antibodies to H. pylori hsp60 in serum is useful for the early monitoring of the effectiveness of eradication therapy.

Original languageEnglish
Pages (from-to)574-577
Number of pages4
JournalClinical and Diagnostic Laboratory Immunology
Volume7
Issue number4
DOIs
Publication statusPublished - Jul 2000

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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