Antibody responses against Porphyromonas gingivalis infection in patients with early-onset periodontitis

Shujuan Guo, Keiso Takahashi, Susumu Kokeguchi, Shogo Takashiba, Denis F. Kinane, Yoji Murayama

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background, aims: The aim of this study was to evaluate antibody responses against Porphyromonas gingivalis (P. gingivalis) infection in early-onset periodontitis (EOP) patients to elucidate further the host-parasite interactions in the pathogenesis of EOP. Method: 16 P. gingivalis-infected EOP and 20 adult periodontitis (AP) patients, and 18 periodontally healthy subjects (HS) participated in this study. Serum immunoglobulin G (IgG) antibody levels and avidities against extracted P. gingivalis whole cells were measured. The components of P. gingivalis outer membrane antigens (OMA) reacting to patients' sera were analysed from the molecular weights by Western blotting. Serum antibody levels against P. gingivalis lipopolysaccharide (LPS) were also measured. The ability of the patients' sera to block interleukin-1 β (IL-1β) production by human mononuclear cells in response to P. gingivalis LPS was examined. Results: Antibody levels were positively correlated with antibody avidities in both EOP and AP patients (r=0.91, r=0.72, p

Original languageEnglish
Pages (from-to)769-777
Number of pages9
JournalJournal of Clinical Periodontology
Volume27
Issue number10
Publication statusPublished - Oct 2000

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Aggressive Periodontitis
Porphyromonas gingivalis
Antibody Formation
Infection
Chronic Periodontitis
Antibody Affinity
Serum
Lipopolysaccharides
Host-Parasite Interactions
Antibodies
Interleukin-1
Healthy Volunteers
Immunoglobulin G
Molecular Weight
Western Blotting
Antigens
Membranes

Keywords

  • Antibody response
  • Early-onset periodontitis
  • Immunodominant antigens
  • Lipopolysaccharide
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Antibody responses against Porphyromonas gingivalis infection in patients with early-onset periodontitis. / Guo, Shujuan; Takahashi, Keiso; Kokeguchi, Susumu; Takashiba, Shogo; Kinane, Denis F.; Murayama, Yoji.

In: Journal of Clinical Periodontology, Vol. 27, No. 10, 10.2000, p. 769-777.

Research output: Contribution to journalArticle

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