Antibodies to human gastric epithelial cells and heat shock protein 60 in Helicobacter pylori positive mucosa associated lymphoid tissue lymphoma

Y. Kawahara, K. Yokota, M. Mizuno, N. Yunoki, T. Uesu, H. Okada, K. Kobayashi, Y. Hirai, K. Oguma, T. Tsuji

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Background - Development of gastric mucosa associated lymphoid tissue (MALT) lymphoma is thought to he closely associated with host immune reactions to Helicobacter pylori. Aim - To investigate humoral immune responses in patients with MALT lymphoma to antigens shared by H pylori and human gastric epithelial cells. Methods - Sera were obtained from H pylori positive patients with MALT lymphoma (n = 11) or other gastroduodenal diseases (peptic ulcer, n = 40; non-ulcer dyspepsia, n = 20) and from H pylori negative healthy control subjects (n = 10). Antibodies to HGC-27 human gastric epithelial cells and human recombinant heat shock protein (Hsp) 60 were examined using an enzyme linked immunosorbent assay (ELISA) and immunoblotting. Results - Antibody titres to HGC-27 cells were significantly elevated in H pylori positive patients with MALT lymphoma when compared with titres in patients with other gastroduodenal diseases and in healthy subjects. Immunoblotting of sera from patients with MALT lymphoma often detected a band with a molecular mass corresponding to Hsp60, and both ELISA and immunoblotting showed elevated antibody titres to the recombinant human Hsp60. Antigenic similarity between Hsp60 and H pylori HspB was documented by immunoblotting experiments. Conclusions - Autoantibodies reactive with host gastric epithelial cells are often increased in MALT lymphoma, and Hsp60 is a major target antigen. Immune responses induced by immunological cross reactivity between H pylori HspB and human Hsp60 in gastric epithelium may be involved in the development of MALT lymphoma.

Original languageEnglish
Pages (from-to)20-23
Number of pages4
JournalGut
Volume45
Issue number1
DOIs
Publication statusPublished - Jan 1 1999

Keywords

  • Autoantibody
  • Heat shock protein
  • Helicobacter pylori
  • Mucosa associated lymphoid tissue lymphoma

ASJC Scopus subject areas

  • Gastroenterology

Fingerprint Dive into the research topics of 'Antibodies to human gastric epithelial cells and heat shock protein 60 in Helicobacter pylori positive mucosa associated lymphoid tissue lymphoma'. Together they form a unique fingerprint.

  • Cite this