Antibodies against heat shock protein 60 derived from Helicobacter pylori: Diagnostic implications in cardiovascular disease

Tomoyuki Okada, Kiyoshi Ayada, Shinichi Usui, Kenji Yokota, Jinhua Cui, Yoshiro Kawahara, Tomoki Inaba, Satoshi Hirohata, Motowo Mizuno, Daisuke Yamamoto, Shozo Kusachi, Eiji Matsuura, Keiji Oguma

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Immune responses against heat shock protein 60 (HSP60) of pathogen-origin are thought to be defensive events which, due to molecular mimicry, misdirect to a human counterpart. Therefore, atherosclerosis may be serologically predicted by anti-HSP60 antibodies (Abs). In the present study, we analyzed the clinical prevalence of the serum IgG Abs against Helicobacter pylori (Hp)-derived HSP60 (Hp-HSP60) or its peptide fragments in patients with cardiovascular disease (CVD; n = 250), as compared to those in age- and gender-matched non-CVD patients (n = 293). Anti-Hp cell lysate Abs frequently appeared in Hp-infected patients who were not associated with CVD. In contrast, Abs against the particular amino acid sequence Hp-HSP60II3 (II3 region, Glu141-Leu160, in Hp-HSP60) predominantly appeared in CVD patients, as well as IgG anti-human HSP60 (Hu-HSP60w). Furthermore, neither titer of anti-Hp-HSP60II3 nor anti-Hu-HSP60w Abs was correlated with the levels of high sensitivity C-reactive protein (hsCRP). This data strongly suggested that IgG anti-Hp-HSP60II3 Abs cross-reacted with Hu-HSP60w were independent diagnostic markers relevant to CVD. Further, the 20 amino acid residues (Glu141-Leu160) might be predominant CVD-associated epitopes that induce anti-Hu-HSP60 auto-Abs, whose location was predicted in the tertiary structure of Hu-HSP60.

Original languageEnglish
Pages (from-to)106-115
Number of pages10
JournalJournal of Autoimmunity
Volume29
Issue number2-3
DOIs
Publication statusPublished - Sep 2007

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Chaperonin 60
Helicobacter pylori
Cardiovascular Diseases
Antibodies
Molecular Mimicry
Peptide Fragments
C-Reactive Protein
Epitopes
Amino Acid Sequence
Atherosclerosis
Immunoglobulin G
Amino Acids
Serum

Keywords

  • Atherosclerosis
  • Cardiovascular disease (CVD)
  • Heat shock protein 60 (HSP60)
  • Helicobacter pylori (Hp)
  • High sensitivity C-reactive protein (hsCRP)

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Antibodies against heat shock protein 60 derived from Helicobacter pylori : Diagnostic implications in cardiovascular disease. / Okada, Tomoyuki; Ayada, Kiyoshi; Usui, Shinichi; Yokota, Kenji; Cui, Jinhua; Kawahara, Yoshiro; Inaba, Tomoki; Hirohata, Satoshi; Mizuno, Motowo; Yamamoto, Daisuke; Kusachi, Shozo; Matsuura, Eiji; Oguma, Keiji.

In: Journal of Autoimmunity, Vol. 29, No. 2-3, 09.2007, p. 106-115.

Research output: Contribution to journalArticle

Okada, Tomoyuki ; Ayada, Kiyoshi ; Usui, Shinichi ; Yokota, Kenji ; Cui, Jinhua ; Kawahara, Yoshiro ; Inaba, Tomoki ; Hirohata, Satoshi ; Mizuno, Motowo ; Yamamoto, Daisuke ; Kusachi, Shozo ; Matsuura, Eiji ; Oguma, Keiji. / Antibodies against heat shock protein 60 derived from Helicobacter pylori : Diagnostic implications in cardiovascular disease. In: Journal of Autoimmunity. 2007 ; Vol. 29, No. 2-3. pp. 106-115.
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