Antibacterial effect of bactericide immobilized in resin matrix

Naoko Namba, Yasuhiro Yoshida, Noriyuki Nagaoka, Seisuke Takashima, Kaori Matsuura-Yoshimoto, Hiroshi Maeda, Bart Van Meerbeek, Kazuomi Suzuki, Shogo Takashiba

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Objective: Biomaterials with anti-microbial properties are highly desirable in the oral cavity. Ideally, bactericidal molecules should be immobilized within the biomaterial to avoid unwanted side-effects against surrounding tissues. They may then however loose much of their antibacterial efficiency. The aim of this study was to investigate how much antibacterial effect an immobilized bactericidal molecule still has against oral bacteria. Methods: Experimental resins containing 0, 1 and 3% cetylpyridinium chloride (CPC) were polymerized, and the bacteriostatic and bactericidal effects against Streptococcus mutans were determined. Adherent S. mutans on HAp was quantitatively determined using FE-SEM and living cells of S. mutans were quantified using real-time RT-PCR. The amount of CPC released from the 0%-, 1%- and 3%-CPC resin sample into water was spectrometrically quantified using a UV-vis recording spectrophotometer. Results: UV spectrometry revealed that less than 0.11 ppm of CPC was released from the resin into water for all specimens, which is lower than the minimal concentration generally needed to inhibit biofilm formation. Growth of S. mutans was significantly inhibited on the surface of the 3%-CPC-containing resin coating, although no inhibitory effect was observed on bacteria that were not in contact with its surface. When immersed in water, the antibacterial capability of 3%-CPC resin lasted for 7 days, as compared to resin that did not contain CPC. Significance: These results demonstrated that the bactericidal molecule still possessed significant contact bacteriostatic activity when it was immobilized in the resin matrix.

Original languageEnglish
Pages (from-to)424-430
Number of pages7
JournalDental Materials
Volume25
Issue number4
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Cetylpyridinium
Bactericides
Resins
Streptococcus mutans
Biocompatible Materials
Biomaterials
Molecules
Water
Bacteria
Spectrophotometers
Biofilms
Spectrometry
Mouth
Real-Time Polymerase Chain Reaction
Spectrum Analysis
Cells
Tissue
Coatings
Scanning electron microscopy
Growth

Keywords

  • Adhesive resin
  • Antibacterial effect
  • Bacteria
  • Bactericide
  • Cetylpyridinium chloride
  • CPC
  • Immobilization
  • Streptococcus mutans

ASJC Scopus subject areas

  • Dentistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Medicine(all)

Cite this

Antibacterial effect of bactericide immobilized in resin matrix. / Namba, Naoko; Yoshida, Yasuhiro; Nagaoka, Noriyuki; Takashima, Seisuke; Matsuura-Yoshimoto, Kaori; Maeda, Hiroshi; Van Meerbeek, Bart; Suzuki, Kazuomi; Takashiba, Shogo.

In: Dental Materials, Vol. 25, No. 4, 04.2009, p. 424-430.

Research output: Contribution to journalArticle

Namba, N, Yoshida, Y, Nagaoka, N, Takashima, S, Matsuura-Yoshimoto, K, Maeda, H, Van Meerbeek, B, Suzuki, K & Takashiba, S 2009, 'Antibacterial effect of bactericide immobilized in resin matrix', Dental Materials, vol. 25, no. 4, pp. 424-430. https://doi.org/10.1016/j.dental.2008.08.012
Namba N, Yoshida Y, Nagaoka N, Takashima S, Matsuura-Yoshimoto K, Maeda H et al. Antibacterial effect of bactericide immobilized in resin matrix. Dental Materials. 2009 Apr;25(4):424-430. https://doi.org/10.1016/j.dental.2008.08.012
Namba, Naoko ; Yoshida, Yasuhiro ; Nagaoka, Noriyuki ; Takashima, Seisuke ; Matsuura-Yoshimoto, Kaori ; Maeda, Hiroshi ; Van Meerbeek, Bart ; Suzuki, Kazuomi ; Takashiba, Shogo. / Antibacterial effect of bactericide immobilized in resin matrix. In: Dental Materials. 2009 ; Vol. 25, No. 4. pp. 424-430.
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abstract = "Objective: Biomaterials with anti-microbial properties are highly desirable in the oral cavity. Ideally, bactericidal molecules should be immobilized within the biomaterial to avoid unwanted side-effects against surrounding tissues. They may then however loose much of their antibacterial efficiency. The aim of this study was to investigate how much antibacterial effect an immobilized bactericidal molecule still has against oral bacteria. Methods: Experimental resins containing 0, 1 and 3{\%} cetylpyridinium chloride (CPC) were polymerized, and the bacteriostatic and bactericidal effects against Streptococcus mutans were determined. Adherent S. mutans on HAp was quantitatively determined using FE-SEM and living cells of S. mutans were quantified using real-time RT-PCR. The amount of CPC released from the 0{\%}-, 1{\%}- and 3{\%}-CPC resin sample into water was spectrometrically quantified using a UV-vis recording spectrophotometer. Results: UV spectrometry revealed that less than 0.11 ppm of CPC was released from the resin into water for all specimens, which is lower than the minimal concentration generally needed to inhibit biofilm formation. Growth of S. mutans was significantly inhibited on the surface of the 3{\%}-CPC-containing resin coating, although no inhibitory effect was observed on bacteria that were not in contact with its surface. When immersed in water, the antibacterial capability of 3{\%}-CPC resin lasted for 7 days, as compared to resin that did not contain CPC. Significance: These results demonstrated that the bactericidal molecule still possessed significant contact bacteriostatic activity when it was immobilized in the resin matrix.",
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