TY - JOUR
T1 - Antibacterial chitosan nanofiber thin films with bacitracin zinc salt
AU - Kumamoto, Kazutaka
AU - Maeda, Toshinari
AU - Hayakawa, Satoshi
AU - Mustapha, Nurul Asyifah Binti
AU - Wang, Meng Jiy
AU - Shirosaki, Yuki
N1 - Funding Information:
Funding: This research was supported by the Japan Society for the Promotion of Science (JSPS) KA-KENHI (grant Number JP19H04471).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/4/1
Y1 - 2021/4/1
N2 - Chitosan nanofiber has a highly uniform structure of 20–50 nm in diameter and shows high dispersibility in water due to its submicron size and high surface-to-volume ratio. The stacked nanofibers film is useful for breathability because it has a gap with a size of several tens of nm or more. However, the chemical bonds between the nanofibers cannot be broken during use. In this study, the thin films were obtained by filtration of chitosan nanofibers and 3-glycidoxypropyltri-methoxysilane (GPTMS) mixture. The addition of GPTMS changed the wettability, mechanical property and stability in water of the thin films. Bacitracin zinc salt (BZ) has been used for the localized dermatological medicines and loaded in the films. BZ interacted electrostatically with the thin films matrix and the release of BZ was controlled by the amount of GPTMS. A higher released amount of BZ showed higher antibacterial effects toward S. aureus. The film was also tested their toxicity by L929 fibroblasts. The release of less than 11.9 μg of BZ showed antibacterial effects, but were not toxic for fibroblast cells.
AB - Chitosan nanofiber has a highly uniform structure of 20–50 nm in diameter and shows high dispersibility in water due to its submicron size and high surface-to-volume ratio. The stacked nanofibers film is useful for breathability because it has a gap with a size of several tens of nm or more. However, the chemical bonds between the nanofibers cannot be broken during use. In this study, the thin films were obtained by filtration of chitosan nanofibers and 3-glycidoxypropyltri-methoxysilane (GPTMS) mixture. The addition of GPTMS changed the wettability, mechanical property and stability in water of the thin films. Bacitracin zinc salt (BZ) has been used for the localized dermatological medicines and loaded in the films. BZ interacted electrostatically with the thin films matrix and the release of BZ was controlled by the amount of GPTMS. A higher released amount of BZ showed higher antibacterial effects toward S. aureus. The film was also tested their toxicity by L929 fibroblasts. The release of less than 11.9 μg of BZ showed antibacterial effects, but were not toxic for fibroblast cells.
KW - Antibacterial properties
KW - Chitosan nanofiber
KW - Fibroblast viability
KW - Thin film
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U2 - 10.3390/polym13071104
DO - 10.3390/polym13071104
M3 - Article
AN - SCOPUS:85103965158
VL - 13
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 7
M1 - 1104
ER -