Antibacterial activity of a novel synthetic progesterone species carrying a linoleic acid molecule against Helicobacter pylori and the hormonal effect of its steroid on a murine macrophage-like cell line

Avarzed Amgalanbaatar, Hirofumi Shimomura, Kouichi Hosoda, Shunji Hayashi, Kenji Yokota, Yoshikazu Hirai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Helicobacter pylori, a pathogen responsible for gastric and duodenal diseases, absorbs various steroid compounds into the cell membrane even though some are toxic to this bacterium. An earlier study by our group has demonstrated that progesterone is bactericidal to H. pylori. In this study, we newly synthesized a steroid compound, 17α-hydroxyprogesterone linoleic acid ester (17hPL), to examine antibacterial activity against H. pylori. As expected, 17hPL acted as a bactericidal agent to H. pylori and had no effect on the survival of other common bacterial species. This steroidal substance interacted with phosphatidylethanolamine (PE) on the outer membrane of H. pylori to induce the release of PE from the bacterial cell membrane and to ultimately lyse the bacterial cells. One of the hormonal effects of progesterone is the inhibition of nitric oxide (NO) production from mouse macrophages activated by lipopolysaccharide (LPS). We therefore examined the inhibition effect of 17hPL on the NO production of RAW 264.7 cells, a murine macrophage-like cell line, stimulated with LPS and demonstrated that 17hPL is relatively weaker in its capability to inhibit NO production in LPS-activated cells than progesterone. These results suggest the possibility that 17hPL could be an oral medicine for selectively treating patients infected with H. pylori.

Original languageEnglish
Pages (from-to)17-25
Number of pages9
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume140
DOIs
Publication statusPublished - 2014

Fingerprint

Macrophages
Linoleic Acid
Helicobacter pylori
Progesterone
Steroids
Cells
Cell Line
Molecules
Lipopolysaccharides
Nitric Oxide
Cell membranes
Cell Membrane
Duodenal Diseases
Poisons
Stomach Diseases
Oral Medicine
Pathogens
Medicine
Bacteria
17-hydroxyprogesterone linoleic acid ester

Keywords

  • 17α- Hydroxyprogesterone
  • 17α-Hydroxyprogesterone caproate
  • 17α-Hydroxyprogesterone linoleic acid ester
  • 2,6-di-O-methyl-β-cyclodextrin
  • Helicobacter pylori
  • Progesterone

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Cell Biology
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Molecular Medicine

Cite this

Antibacterial activity of a novel synthetic progesterone species carrying a linoleic acid molecule against Helicobacter pylori and the hormonal effect of its steroid on a murine macrophage-like cell line. / Amgalanbaatar, Avarzed; Shimomura, Hirofumi; Hosoda, Kouichi; Hayashi, Shunji; Yokota, Kenji; Hirai, Yoshikazu.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 140, 2014, p. 17-25.

Research output: Contribution to journalArticle

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