TY - JOUR
T1 - Antibacterial activity and biocompability of zinc oxide and graphite particles as endodontic materials
AU - Kozuszko, Silvia Noemí
AU - Sánchez, María Alejandra
AU - de Ferro, María Inés Gutiérrez
AU - Sfer, Ana María
AU - Madrid, Ana Paula Moreno
AU - Takabatake, Kiyofumi
AU - Nakano, Keisuke
AU - Nagatsuka, Hitoshi
AU - Rodríguez, Andrea Paola
N1 - Funding Information:
Financial support from National Agency for Scientific and Technological Promotion (PDTS 2016 N?574) is acknowledged. Also this study is jointly funded by the Japan Society for Promotion of Science (JSPS) KAKENHI Grant-in-Aid for Scientific Research (16K11441) and (16K20577). Further, authors would like to thank Eng. B. Felice for correcting the language of this paper and Dr. Susana Gutierrez from Dentistry Faculty for providing the Staph. aureus strain and for her advice.
Publisher Copyright:
© 2017, Society of Hard Tissue Regenerative Biology. All rights reserved.
PY - 2017
Y1 - 2017
N2 - The aim of this work is to evaluate the antibacterial effect and biocompatibility of zinc oxide (ZnO) nanopaticles and graphite-type carbon (Gt) microparticles commercial powders. SEM analysis was performed to assess particles morphology. The antibacterial behavior was studied against Staphylococcus aureus (Staph. Aureus) (bacterial strain ATCC 29213) and TEM analysis of bacteria was performed to determine ultrastructural alterations; in addition, biocompatibility was evaluated in subcutaneous tissue of Wistar rats at 3, 7 and 28 d. ZnO and Gt powders exhibited antibacterial activity while TEM images of Staph. aureus showed membrane disruption followed by the release of internal content. Also, an electron-light region within the cytoplasm was observed for microorganisms treated with ZnO. Regarding biocompatibility, Gt samples induced a foreign body reaction response with presence of giant cells whereas ZnO samples showed fibroblastic connective tissue with chronic inflammatory cells and new small vessels. Also, collagen fibers and lack of capsule was observed by Trichome Masson stain. Thus, ZnO improved wound healing by enhancing tissue regeneration in contrast with calcium hydroxide control sample response which showed a fibrous tissue scar. Hence, ZnO nano-powder seems to be a potential material in the regenerative endodontic field.
AB - The aim of this work is to evaluate the antibacterial effect and biocompatibility of zinc oxide (ZnO) nanopaticles and graphite-type carbon (Gt) microparticles commercial powders. SEM analysis was performed to assess particles morphology. The antibacterial behavior was studied against Staphylococcus aureus (Staph. Aureus) (bacterial strain ATCC 29213) and TEM analysis of bacteria was performed to determine ultrastructural alterations; in addition, biocompatibility was evaluated in subcutaneous tissue of Wistar rats at 3, 7 and 28 d. ZnO and Gt powders exhibited antibacterial activity while TEM images of Staph. aureus showed membrane disruption followed by the release of internal content. Also, an electron-light region within the cytoplasm was observed for microorganisms treated with ZnO. Regarding biocompatibility, Gt samples induced a foreign body reaction response with presence of giant cells whereas ZnO samples showed fibroblastic connective tissue with chronic inflammatory cells and new small vessels. Also, collagen fibers and lack of capsule was observed by Trichome Masson stain. Thus, ZnO improved wound healing by enhancing tissue regeneration in contrast with calcium hydroxide control sample response which showed a fibrous tissue scar. Hence, ZnO nano-powder seems to be a potential material in the regenerative endodontic field.
KW - Antibacterial agents
KW - Foreign body reaction
KW - Graphite
KW - Staphylococcus aureus
KW - Zinc oxide
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U2 - 10.2485/jhtb.26.311
DO - 10.2485/jhtb.26.311
M3 - Article
AN - SCOPUS:85030647279
SN - 1341-7649
VL - 26
SP - 311
EP - 318
JO - Journal of Hard Tissue Biology
JF - Journal of Hard Tissue Biology
IS - 4
ER -