Antibacterial action of several tannins against Staphylococcus aureus

H. Akiyama, K. Fujii, Osamu Yamasaki, Takashi Oono, K. Iwatsuki

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Abstract

We examined the antibacterial action of several tannins on plasma coagulation by Staphylococcus aureus and the effect of conventional chemotherapy combined with tannic acid below the MIC. Coagulation was inhibited in plasma containing tannic acid (100 mg/L), gallic acid (5000 mg/L), ellagic acid (5000 mg/L), (-)-epicatechin (1500 mg/L), (-)-epicatechin gallate (500 mg/L) or (-)-epigallocatechin gallate (200 mg/L) after incubation for 24 h. All tannins inhibited coagulation at a concentration below the MIC. The MICs of oxacillin and cefdinir for S. aureus were reduced to ≤ 0.06 mg/L in Mueller-Hinton agar plates with tannic acid (100 mg/L) at a concentration below the MIC. The antistaphylococcal activity of tannic acid was reduced in plates with 10% rabbit blood, but not in those with 10% rabbit plasma. Membranous structures formed in a culture medium containing equal proportions of plasma and tryptic soy broth after incubation for 24 h. The colony counts of S. aureus in membranous structures in the medium containing oxacillin (40 mg/L) and tannic acid (100 mg/L) were c. 10-fold lower than those in medium containing oxacillin (40 mg/L) alone (P <0.01). Tannic acid merits further investigation as a possible adjuvant agent against S. aureus skin infections treated with β-lactam antibiotics.

Original languageEnglish
Pages (from-to)487-491
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume48
Issue number4
Publication statusPublished - 2001

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Tannins
Staphylococcus aureus
Oxacillin
cefdinir
Ellagic Acid
Rabbits
Lactams
Gallic Acid
Catechin
Agar
Culture Media
Anti-Bacterial Agents
Drug Therapy
Skin
Infection

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology

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Antibacterial action of several tannins against Staphylococcus aureus. / Akiyama, H.; Fujii, K.; Yamasaki, Osamu; Oono, Takashi; Iwatsuki, K.

In: Journal of Antimicrobial Chemotherapy, Vol. 48, No. 4, 2001, p. 487-491.

Research output: Contribution to journalArticle

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