Anti-P-selectin antibody attenuates rat brain ischemic injury

H. Suzuki, T. Hayashi, S. J. Tojo, H. Kitagawa, K. Kimura, M. Mizugaki, Y. Itoyama, K. Abe

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

We examined a protective effect of anti-P-selectin monoclonal antibody against rat ischemic brain injury with 24 h of middle cerebral artery occlusion (MCAO). Anti-rat P-selectin monoclonal antibody, was intravenously injected at a dose of 1 mg/kg at 5 min before MCAO. Control animals received the same volume of vehicle. MCAO was accomplished by an insertion of nylon thread with silicone coating for 24 h. Application of anti-P-selectin antibody significantly reduced infarct size and brain water content at 24 h of MCAO. Although leukocyte infiltration was not normally detected, it became remarkably evident at 1 day of MCAO. However, treatment with ARP 2-4 significantly reduced the number of leukocytes. These results demonstrated that administration of monoclonal antibody against P-selectin attenuated infarct size and brain edema, which was associated with reduction of leukocyte infiltration.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalNeuroscience Letters
Volume265
Issue number3
DOIs
Publication statusPublished - Apr 23 1999

Keywords

  • Brain infarct
  • Cerebral ischemia
  • Edema
  • Middle cerebral artery
  • P-selectin
  • Rats

ASJC Scopus subject areas

  • Neuroscience(all)

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