Anti-P-selectin antibody attenuates rat brain ischemic injury

H. Suzuki, T. Hayashi, S. J. Tojo, H. Kitagawa, K. Kimura, M. Mizugaki, Y. Itoyama, Koji Abe

Research output: Contribution to journalArticle

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Abstract

We examined a protective effect of anti-P-selectin monoclonal antibody against rat ischemic brain injury with 24 h of middle cerebral artery occlusion (MCAO). Anti-rat P-selectin monoclonal antibody, was intravenously injected at a dose of 1 mg/kg at 5 min before MCAO. Control animals received the same volume of vehicle. MCAO was accomplished by an insertion of nylon thread with silicone coating for 24 h. Application of anti-P-selectin antibody significantly reduced infarct size and brain water content at 24 h of MCAO. Although leukocyte infiltration was not normally detected, it became remarkably evident at 1 day of MCAO. However, treatment with ARP 2-4 significantly reduced the number of leukocytes. These results demonstrated that administration of monoclonal antibody against P-selectin attenuated infarct size and brain edema, which was associated with reduction of leukocyte infiltration.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalNeuroscience Letters
Volume265
Issue number3
DOIs
Publication statusPublished - Apr 23 1999

Fingerprint

P-Selectin
Middle Cerebral Artery Infarction
Brain Injuries
Antibodies
Monoclonal Antibodies
Leukocytes
Nylons
Brain Edema
Silicones
Leukocyte Count
Water
Brain

Keywords

  • Brain infarct
  • Cerebral ischemia
  • Edema
  • Middle cerebral artery
  • P-selectin
  • Rats

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Suzuki, H., Hayashi, T., Tojo, S. J., Kitagawa, H., Kimura, K., Mizugaki, M., ... Abe, K. (1999). Anti-P-selectin antibody attenuates rat brain ischemic injury. Neuroscience Letters, 265(3), 163-166. https://doi.org/10.1016/S0304-3940(99)00229-3

Anti-P-selectin antibody attenuates rat brain ischemic injury. / Suzuki, H.; Hayashi, T.; Tojo, S. J.; Kitagawa, H.; Kimura, K.; Mizugaki, M.; Itoyama, Y.; Abe, Koji.

In: Neuroscience Letters, Vol. 265, No. 3, 23.04.1999, p. 163-166.

Research output: Contribution to journalArticle

Suzuki, H, Hayashi, T, Tojo, SJ, Kitagawa, H, Kimura, K, Mizugaki, M, Itoyama, Y & Abe, K 1999, 'Anti-P-selectin antibody attenuates rat brain ischemic injury', Neuroscience Letters, vol. 265, no. 3, pp. 163-166. https://doi.org/10.1016/S0304-3940(99)00229-3
Suzuki H, Hayashi T, Tojo SJ, Kitagawa H, Kimura K, Mizugaki M et al. Anti-P-selectin antibody attenuates rat brain ischemic injury. Neuroscience Letters. 1999 Apr 23;265(3):163-166. https://doi.org/10.1016/S0304-3940(99)00229-3
Suzuki, H. ; Hayashi, T. ; Tojo, S. J. ; Kitagawa, H. ; Kimura, K. ; Mizugaki, M. ; Itoyama, Y. ; Abe, Koji. / Anti-P-selectin antibody attenuates rat brain ischemic injury. In: Neuroscience Letters. 1999 ; Vol. 265, No. 3. pp. 163-166.
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