Anti-oxidative and anti-inflammatory effects of spirulina on rat model of non-alcoholic steatohepatitis

Wing Pak, Fusako Takayama, Manaka Mine, Kazuo Nakamoto, Yasumasa Kodo, Mitsumasa Mankura, Toru Egashira, Hiromu Kawasaki, Akitane Mori

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The pathogeneneis of nonalcoholic steatohepatitis (NASH) remains unclear, but accumulating data suggest oxidative stress and the relationship between inflammation and immunity plays a crucial role. The aim of this study is to investigate the spirulina, which is a blue-green algae rich in proteins and other nutritional elements, and its component-phycocyanin effect on a rat model of NASH. NASH model rats were established by feeding male Wistar rats with choline-deficient high-fat diet (CDHF) and intermittent hypoxemia by sodium nitrite challenge after 5 weeks of CDHF. After experimental period of 10 weeks, blood and liver were collected to determine oxidative stress injuries and efficacies of spirulina or phycocyanin on NASH model rats. In the NASH model rats, increase in plasma liver enzymes and liver fibrosis, increases in productions of reactive oxygen species from liver mitochondria and from leukocytes, the activation of nuclear factor-kappa B, and the change in the lymphocyte surface antigen ratio (CD4+/CD8+) were observed. The spirulina and phycocyanin administration significantly abated these changes. The spirulina or phycocyanin administration to model rats of NASH might lessen the inflammatory response through anti-oxidative and anti-inflammatory mechanisms, breaking the crosstalk between oxidative stress and inflammation, and effectively inhibit NASH progression.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalJournal of Clinical Biochemistry and Nutrition
Volume51
Issue number3
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Spirulina
Fatty Liver
Phycocyanin
Rats
Anti-Inflammatory Agents
Liver
Oxidative stress
Nutrition
Oxidative Stress
Choline
High Fat Diet
Fats
Sodium Nitrite
Mitochondria
Lymphocytes
Inflammation
NF-kappa B
CD4-CD8 Ratio
Surface Antigens
Crosstalk

Keywords

  • Anti-inflammation
  • Anti-oxidant
  • Non-alcoholic steatohepatitis
  • Oxidative stress
  • Spirulina

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Anti-oxidative and anti-inflammatory effects of spirulina on rat model of non-alcoholic steatohepatitis. / Pak, Wing; Takayama, Fusako; Mine, Manaka; Nakamoto, Kazuo; Kodo, Yasumasa; Mankura, Mitsumasa; Egashira, Toru; Kawasaki, Hiromu; Mori, Akitane.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 51, No. 3, 11.2012, p. 227-234.

Research output: Contribution to journalArticle

Pak, Wing ; Takayama, Fusako ; Mine, Manaka ; Nakamoto, Kazuo ; Kodo, Yasumasa ; Mankura, Mitsumasa ; Egashira, Toru ; Kawasaki, Hiromu ; Mori, Akitane. / Anti-oxidative and anti-inflammatory effects of spirulina on rat model of non-alcoholic steatohepatitis. In: Journal of Clinical Biochemistry and Nutrition. 2012 ; Vol. 51, No. 3. pp. 227-234.
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