Anti-osteoporosis effects of 1,4-dihydroxy-2-naphthoic acid in ovariectomized mice with increasing of bone density

Kenichiro Kita, Eiki Yamachika, Masakazu Matsubara, Hidetsugu Tsujigiwa, Nobuhisa Ishida, Norifumi Moritani, Tatsushi Matsumura, Masahide Mizutani, Yuki Fujita, Kiyofumi Takabatake, Kenichiro Ejima, Hitoshi Nagatsuka, Yoshinori Yamaguchi, Seiji Iida

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: 1,4-Dihydroxy-2-naphthoic acid (DHNA) is the main component of the metabolic products after fermentation by Propionibacterium freudenreichii ET-3. In this study, DHNA, vitamin K2 (VK2), and risedronate (Ris) were administered to ovariectomized (OVX) mice to ascertain their suppressive effects on bone mass reduction. Methods: Fifty mice were divided into five groups: Sham, OVX, DHNA, VK2, and Ris groups. The drugs were administered daily for 8 weeks. Subsequently, blood was collected from the orbital venous plexus. The bilateral femurs and L5 lumbar vertebra were excised. To determine the effects of the drugs, we performed histomorphometric analyses of the left femur and L5 by micro-CT, biochemical analyses of serum samples, and histological analyses of the right femur. Results: The results for the total bone mineral density, bone volume density, trabecular thickness, trabecular number, trabecular separation, and histological analyses showed that bone resorption was improved in the DHNA and Ris groups compared with the OVX group. Furthermore, the biochemical analyses revealed that the production of inflammatory cytokines was suppressed in the DHNA and Ris groups. Conclusions: The present findings show that DHNA suppresses the bone mass reduction in OVX mice, suggesting that it could be an alternative treatment for postmenopausal osteoporosis.

Original languageEnglish
Pages (from-to)66-72
Number of pages7
JournalJournal of Oral and Maxillofacial Surgery, Medicine, and Pathology
Volume28
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

Keywords

  • Bone resorption
  • DHNA
  • Osteoporosis
  • Ovariectomized mice
  • Risedronate

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Pathology and Forensic Medicine
  • Otorhinolaryngology

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