Anti-laminin-1 autoantibodies, pregnancy loss and endometriosis

Junko Inagaki, Akane Kondo, Luis R. Lopez, Yehuda Shoenfeld, Eiji Matsuura

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Laminin-1 is a major component and multifunctional glycoprotein of basement membranes that consists of three different subunits, α1, β1 and γ1 chains. It is the earliest synthesized network-forming protein during embryogenesis and plays an important role in embryonic development, embryonic implantation and placentation. We have recently shown that IgG anti-laminin-1 antibodies were significantly associated with recurrent first-trimester miscarriages and with subsequent pregnancy outcome. Interestingly, these antibodies were also observed in patients with endometriosis-associated infertility but not in patients with other causes of infertility, including tubal factors, hormonal and uterine abnormalities. Laminin-α1, -β1 and -γ1 mRNAs have been detected in 90% of endometriotic lesions and all laminin-α1, -β1 and -γ1 chains were localized in the basement membranes of glandular epithelium in endometriotic peritoneal lesions. Western blot analysis showed that anti-laminin-1 antibodies from those patients reacted with all laminin-1's chains. ELISA also confirmed that one of the target epitopes for these antibodies was located in a particular region of the laminin-1 molecule, i.e. the carboxyl-terminal globular G domain of α1 chain. IgM monoclonal anti-laminin-1 autoantibody, that we recently established, also recognized the G domain. Anti-laminin-1 antibodies from mice immunized with "mouse" laminin-1, caused a higher fetal resorption rate with lower embryonic and placental weights. Thus, anti-laminin-1 antibodies may be important in development of autoimmune-mediated reproductive failures and the assessment of the antibodies may provide a novel non-invasive diagnosis of endometriosis.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalClinical and Developmental Immunology
Volume11
Issue number3-4
DOIs
Publication statusPublished - Sep 2004

Fingerprint

Endometriosis
Autoantibodies
Pregnancy
Antibodies
Basement Membrane
Infertility
Embryonic Development
laminin 1
Fetal Resorption
Placentation
Spontaneous Abortion
First Pregnancy Trimester
Pregnancy Outcome
Epitopes
Glycoproteins
Epithelium
Western Blotting
Enzyme-Linked Immunosorbent Assay
Weights and Measures
Messenger RNA

Keywords

  • Anti-laminin-1 autoantibody
  • Endometriosis
  • Fetal loss
  • Infertility
  • Recurrent abortion

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Developmental Biology

Cite this

Anti-laminin-1 autoantibodies, pregnancy loss and endometriosis. / Inagaki, Junko; Kondo, Akane; Lopez, Luis R.; Shoenfeld, Yehuda; Matsuura, Eiji.

In: Clinical and Developmental Immunology, Vol. 11, No. 3-4, 09.2004, p. 261-266.

Research output: Contribution to journalArticle

Inagaki, Junko ; Kondo, Akane ; Lopez, Luis R. ; Shoenfeld, Yehuda ; Matsuura, Eiji. / Anti-laminin-1 autoantibodies, pregnancy loss and endometriosis. In: Clinical and Developmental Immunology. 2004 ; Vol. 11, No. 3-4. pp. 261-266.
@article{ab685b21849142aa900e432be20fc0c9,
title = "Anti-laminin-1 autoantibodies, pregnancy loss and endometriosis",
abstract = "Laminin-1 is a major component and multifunctional glycoprotein of basement membranes that consists of three different subunits, α1, β1 and γ1 chains. It is the earliest synthesized network-forming protein during embryogenesis and plays an important role in embryonic development, embryonic implantation and placentation. We have recently shown that IgG anti-laminin-1 antibodies were significantly associated with recurrent first-trimester miscarriages and with subsequent pregnancy outcome. Interestingly, these antibodies were also observed in patients with endometriosis-associated infertility but not in patients with other causes of infertility, including tubal factors, hormonal and uterine abnormalities. Laminin-α1, -β1 and -γ1 mRNAs have been detected in 90{\%} of endometriotic lesions and all laminin-α1, -β1 and -γ1 chains were localized in the basement membranes of glandular epithelium in endometriotic peritoneal lesions. Western blot analysis showed that anti-laminin-1 antibodies from those patients reacted with all laminin-1's chains. ELISA also confirmed that one of the target epitopes for these antibodies was located in a particular region of the laminin-1 molecule, i.e. the carboxyl-terminal globular G domain of α1 chain. IgM monoclonal anti-laminin-1 autoantibody, that we recently established, also recognized the G domain. Anti-laminin-1 antibodies from mice immunized with {"}mouse{"} laminin-1, caused a higher fetal resorption rate with lower embryonic and placental weights. Thus, anti-laminin-1 antibodies may be important in development of autoimmune-mediated reproductive failures and the assessment of the antibodies may provide a novel non-invasive diagnosis of endometriosis.",
keywords = "Anti-laminin-1 autoantibody, Endometriosis, Fetal loss, Infertility, Recurrent abortion",
author = "Junko Inagaki and Akane Kondo and Lopez, {Luis R.} and Yehuda Shoenfeld and Eiji Matsuura",
year = "2004",
month = "9",
doi = "10.1080/17402520400001678",
language = "English",
volume = "11",
pages = "261--266",
journal = "Journal of Immunology Research",
issn = "2314-8861",
publisher = "Hindawi Publishing Corporation",
number = "3-4",

}

TY - JOUR

T1 - Anti-laminin-1 autoantibodies, pregnancy loss and endometriosis

AU - Inagaki, Junko

AU - Kondo, Akane

AU - Lopez, Luis R.

AU - Shoenfeld, Yehuda

AU - Matsuura, Eiji

PY - 2004/9

Y1 - 2004/9

N2 - Laminin-1 is a major component and multifunctional glycoprotein of basement membranes that consists of three different subunits, α1, β1 and γ1 chains. It is the earliest synthesized network-forming protein during embryogenesis and plays an important role in embryonic development, embryonic implantation and placentation. We have recently shown that IgG anti-laminin-1 antibodies were significantly associated with recurrent first-trimester miscarriages and with subsequent pregnancy outcome. Interestingly, these antibodies were also observed in patients with endometriosis-associated infertility but not in patients with other causes of infertility, including tubal factors, hormonal and uterine abnormalities. Laminin-α1, -β1 and -γ1 mRNAs have been detected in 90% of endometriotic lesions and all laminin-α1, -β1 and -γ1 chains were localized in the basement membranes of glandular epithelium in endometriotic peritoneal lesions. Western blot analysis showed that anti-laminin-1 antibodies from those patients reacted with all laminin-1's chains. ELISA also confirmed that one of the target epitopes for these antibodies was located in a particular region of the laminin-1 molecule, i.e. the carboxyl-terminal globular G domain of α1 chain. IgM monoclonal anti-laminin-1 autoantibody, that we recently established, also recognized the G domain. Anti-laminin-1 antibodies from mice immunized with "mouse" laminin-1, caused a higher fetal resorption rate with lower embryonic and placental weights. Thus, anti-laminin-1 antibodies may be important in development of autoimmune-mediated reproductive failures and the assessment of the antibodies may provide a novel non-invasive diagnosis of endometriosis.

AB - Laminin-1 is a major component and multifunctional glycoprotein of basement membranes that consists of three different subunits, α1, β1 and γ1 chains. It is the earliest synthesized network-forming protein during embryogenesis and plays an important role in embryonic development, embryonic implantation and placentation. We have recently shown that IgG anti-laminin-1 antibodies were significantly associated with recurrent first-trimester miscarriages and with subsequent pregnancy outcome. Interestingly, these antibodies were also observed in patients with endometriosis-associated infertility but not in patients with other causes of infertility, including tubal factors, hormonal and uterine abnormalities. Laminin-α1, -β1 and -γ1 mRNAs have been detected in 90% of endometriotic lesions and all laminin-α1, -β1 and -γ1 chains were localized in the basement membranes of glandular epithelium in endometriotic peritoneal lesions. Western blot analysis showed that anti-laminin-1 antibodies from those patients reacted with all laminin-1's chains. ELISA also confirmed that one of the target epitopes for these antibodies was located in a particular region of the laminin-1 molecule, i.e. the carboxyl-terminal globular G domain of α1 chain. IgM monoclonal anti-laminin-1 autoantibody, that we recently established, also recognized the G domain. Anti-laminin-1 antibodies from mice immunized with "mouse" laminin-1, caused a higher fetal resorption rate with lower embryonic and placental weights. Thus, anti-laminin-1 antibodies may be important in development of autoimmune-mediated reproductive failures and the assessment of the antibodies may provide a novel non-invasive diagnosis of endometriosis.

KW - Anti-laminin-1 autoantibody

KW - Endometriosis

KW - Fetal loss

KW - Infertility

KW - Recurrent abortion

UR - http://www.scopus.com/inward/record.url?scp=7044224770&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7044224770&partnerID=8YFLogxK

U2 - 10.1080/17402520400001678

DO - 10.1080/17402520400001678

M3 - Article

C2 - 15559372

AN - SCOPUS:7044224770

VL - 11

SP - 261

EP - 266

JO - Journal of Immunology Research

JF - Journal of Immunology Research

SN - 2314-8861

IS - 3-4

ER -