Anti-inflammatory effect of linear polarized infrared irradiation on interleukin-1β-induced chemokine production in MH7A rheumatoid synovial cells

Yasuko Shibata, Naomi Ogura, Keisuke Yamashiro, Shogo Takashiba, Toshirou Kondoh, Keiji Miyazawa, Masaru Matsui, Yoshimitsu Abiko

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We examined the anti-inflammatory effect of infrared linear polarized light irradiation on the MH7A rheumatoid fibroblast-like synoviocytes (FLS) stimulated with the proinflammatory cytokine interleukin (IL)-1β. Expression of messenger ribonucleic acids (mRNAs) encoding IL-8, RANTES (regulated upon activation, normal T cell expressed and secreted), growth-related gene alpha (GROα), and macrophage inflammatory protein-1α (MIP1α) was measured using real-time reverse transcription polymerase chain reaction, and the secreted proteins were measured in the conditioned media using enzyme-linked immunosorbent assays. We found that irradiation with linear polarized infrared light suppressed IL-1β-induced expression of IL-8 mRNA and, correspondingly, the synthesis and release of IL-8 protein in MH7A cells. This anti-inflammatory effect was equivalent to that obtained with the glucocorticoid dexamethasone. Likewise, irradiation suppressed the IL-1β-induced expression of RANTES and GROα mRNA. These results suggest that the irradiation of the areas around the articular surfaces of joints affected by rheumatoid arthritis (RA) using linear polarized light may represent a useful new approach to treatment.

Original languageEnglish
Pages (from-to)109-113
Number of pages5
JournalLasers in Medical Science
Volume20
Issue number3-4
DOIs
Publication statusPublished - Dec 2005

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Interleukin-8
Interleukin-1
Chemokines
Anti-Inflammatory Agents
RNA
Light
Joints
Chemokine CCL3
T-Lymphocytes
Conditioned Culture Medium
Dexamethasone
Glucocorticoids
Reverse Transcription
Rheumatoid Arthritis
Proteins
Fibroblasts
Enzyme-Linked Immunosorbent Assay
Cytokines
Polymerase Chain Reaction
Growth

Keywords

  • Dexamethasone
  • IL-1β
  • IL-8
  • Linear polarized light
  • Rheumatoid arthritis synoviocytes MH7A
  • Super lizer

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Surgery

Cite this

Anti-inflammatory effect of linear polarized infrared irradiation on interleukin-1β-induced chemokine production in MH7A rheumatoid synovial cells. / Shibata, Yasuko; Ogura, Naomi; Yamashiro, Keisuke; Takashiba, Shogo; Kondoh, Toshirou; Miyazawa, Keiji; Matsui, Masaru; Abiko, Yoshimitsu.

In: Lasers in Medical Science, Vol. 20, No. 3-4, 12.2005, p. 109-113.

Research output: Contribution to journalArticle

Shibata, Yasuko ; Ogura, Naomi ; Yamashiro, Keisuke ; Takashiba, Shogo ; Kondoh, Toshirou ; Miyazawa, Keiji ; Matsui, Masaru ; Abiko, Yoshimitsu. / Anti-inflammatory effect of linear polarized infrared irradiation on interleukin-1β-induced chemokine production in MH7A rheumatoid synovial cells. In: Lasers in Medical Science. 2005 ; Vol. 20, No. 3-4. pp. 109-113.
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