Anti-inflammatory effect of arginase inhibitor and corticosteroid on airway allergic reactions in a dermatophogoides farinae-induced NC/Nga mouse model

Keiki Ogino, Masayuki Kubo, Hidekazu Takahashi, Ran Zhang, Yu Zou, Yoshihisa Fujikura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The present study was aimed to investigate the effect of an arginase inhibitor, N-hydroxy-nor-l-Arginine (nor-NOHA) and a corticosteroid, prednisolone, in an intranasal mite-induced NC/Nga mouse model of asthma. The treatment with nor-NOHA and prednisolone inhibited the increase in airway hyperresponsiveness, the number of bronchoalveolar lavage fluid cells, protein expression of arginase I and arginase II, messenger RNA (mRNA) expression of nitric oxide synthase (NOS)2 and Th2 cytokines such as interleukin (IL)-4, IL-5, and IL-13, and the pathological inflammatory changes of the lung. NOx levels in the lung were not changed in mice treated with prednisolone and elevated in mice treated with nor-NOHA or prednisolone plus nor-NOHA despite suppressed NOS2 mRNA expression. The study concluded that anti-inflammatory effect by nor-NOHA might be dependent on NO supply from depleted NO by downregulated arginine availability of arginase and was not related with the anti-inflammatory mechanisms by prednisolone.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalInflammation
Volume36
Issue number1
DOIs
Publication statusPublished - Feb 2013
Externally publishedYes

Keywords

  • arginase inhibitor
  • corticosteroid
  • experimental asthma
  • nitric oxide

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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