Anti-inflammatory effect of arginase inhibitor and corticosteroid on airway allergic reactions in a dermatophogoides farinae-induced NC/Nga mouse model

Keiki Ogino, Masayuki Kubo, Hidekazu Takahashi, Ran Zhang, Yu Zou, Yoshihisa Fujikura

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The present study was aimed to investigate the effect of an arginase inhibitor, N-hydroxy-nor-l-Arginine (nor-NOHA) and a corticosteroid, prednisolone, in an intranasal mite-induced NC/Nga mouse model of asthma. The treatment with nor-NOHA and prednisolone inhibited the increase in airway hyperresponsiveness, the number of bronchoalveolar lavage fluid cells, protein expression of arginase I and arginase II, messenger RNA (mRNA) expression of nitric oxide synthase (NOS)2 and Th2 cytokines such as interleukin (IL)-4, IL-5, and IL-13, and the pathological inflammatory changes of the lung. NOx levels in the lung were not changed in mice treated with prednisolone and elevated in mice treated with nor-NOHA or prednisolone plus nor-NOHA despite suppressed NOS2 mRNA expression. The study concluded that anti-inflammatory effect by nor-NOHA might be dependent on NO supply from depleted NO by downregulated arginine availability of arginase and was not related with the anti-inflammatory mechanisms by prednisolone.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalInflammation
Volume36
Issue number1
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Arginase
Prednisolone
Adrenal Cortex Hormones
Hypersensitivity
Anti-Inflammatory Agents
Arginine
Lung
Messenger RNA
Interleukin-13
Mites
Interleukin-5
Bronchoalveolar Lavage Fluid
Nitric Oxide Synthase
Interleukin-4
Down-Regulation
Asthma
Cytokines
Proteins

Keywords

  • arginase inhibitor
  • corticosteroid
  • experimental asthma
  • nitric oxide

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Anti-inflammatory effect of arginase inhibitor and corticosteroid on airway allergic reactions in a dermatophogoides farinae-induced NC/Nga mouse model. / Ogino, Keiki; Kubo, Masayuki; Takahashi, Hidekazu; Zhang, Ran; Zou, Yu; Fujikura, Yoshihisa.

In: Inflammation, Vol. 36, No. 1, 02.2013, p. 141-151.

Research output: Contribution to journalArticle

Ogino, Keiki ; Kubo, Masayuki ; Takahashi, Hidekazu ; Zhang, Ran ; Zou, Yu ; Fujikura, Yoshihisa. / Anti-inflammatory effect of arginase inhibitor and corticosteroid on airway allergic reactions in a dermatophogoides farinae-induced NC/Nga mouse model. In: Inflammation. 2013 ; Vol. 36, No. 1. pp. 141-151.
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