Anti-Inflammatory Effect of Amlodipine Plus Atorvastatin Treatment on Carotid Atherosclerosis in Zucker Metabolic Syndrome Rats

Xuemei Zhang, Fengfeng Tian, Hiromi Kawai, Tomoko Kurata, Shoko Deguchi, Kentaro Deguchi, Jingwei Shang, Ning Liu, Wentao Liu, Yoshio Ikeda, Tohru Matsuura, Tatsushi Kamiya, Koji Abe

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3 Citations (Scopus)

Abstract

To investigate the effects of amlodipine in combination with atorvastatin on carotid atherosclerotic changes in metabolic syndrome, 8-week-old Zucker fatty rats were treated with vehicle, amlodipine, atorvastatin, or amlodipine in combination with atorvastatin for 28 days. Histological studies of common carotid arteries showed that lipid deposition determined by Sudan III staining was significantly reduced in rats treated with amlodipine or atorvastatin alone and was further reduced by amlodipine in combination with atorvastatin. Immunohistochemical studies of the pro-inflammatory cytokine tumor necrosis factor (TNF)-α, the arterial calcification initiator bone morphogenetic protein (BMP) 2, the angiogenic factor Notch1, and the smooth muscle cell marker α-smooth muscle actin (SMA) showed that the high expression of all four protein in vehicle-treated rats was greatly decreased by amlodipine, atorvastatin, or amlodipine in combination with atorvastatin, in ascending order. Double immunostaining showed marked colocalization of TNF-α with bone morphogenetic protein 2 and Notch1 with α-SMA in the vehicle group, which was greatly reduced by amlodipine plus atorvastatin. These data suggest that combination therapy may be more effective in preventing atherosclerotic processes and subsequent carotid vascular events than administrating amlodipine or atorvastatin alone in metabolic syndrome.

Original languageEnglish
Pages (from-to)435-441
Number of pages7
JournalTranslational Stroke Research
Volume3
Issue number4
DOIs
Publication statusPublished - Dec 2012

Keywords

  • Amlodipine
  • Atorvastatin
  • Common carotid artery
  • Zucker metabolic syndrome rat

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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    Zhang, X., Tian, F., Kawai, H., Kurata, T., Deguchi, S., Deguchi, K., Shang, J., Liu, N., Liu, W., Ikeda, Y., Matsuura, T., Kamiya, T., & Abe, K. (2012). Anti-Inflammatory Effect of Amlodipine Plus Atorvastatin Treatment on Carotid Atherosclerosis in Zucker Metabolic Syndrome Rats. Translational Stroke Research, 3(4), 435-441. https://doi.org/10.1007/s12975-012-0198-1