Anti-inflammatory and intestinal barrier-protective activities of commensal lactobacilli and bifidobacteria in thoroughbreds: Role of probiotics in diarrhea prevention in neonatal Thoroughbreds

Soichi Tanabe, Takuya Suzuki, Yuichiro Wasano, Fumihiko Nakajima, Hiroshi Kawasaki, Tomonori Tsuda, Natsuko Nagamine, Takashi Tsurumachi, Kiyoshi Sugaya, Hiroaki Akita, Misako Takagi, Kunihiko Takagi, Yoshinobu Inoue, Yo Asai, Hidetoshi Morita

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We previously isolated the commensal bacteria lactobacilli and bifidobacteria from the Thoroughbred intestine and prepared the horse probiotics LacFi™, consisting of Lactobacillus ruminis KK14, L. equi KK 15, L. reuteri KK18, L. johnsonii KK21, and Bifidobacterium boum HU. Here, we found that the fve LacFi™ constituent strains remarkably suppressed pro-inflammatory interleukin-17 production in mouse splenocytes stimulated with interleukin-6 and transforming growth factor-β. The protective effects of the probiotic on impaired intestinal barrier function were evaluated in Caco-2 cells treated with tumor necrosis factor-α. Evaluation of transepithelial resistance showed that all the strains exhibited intestinal barrier protective activity, with significant suppression of barrier impairment by L. reuteri KK18. The LacFi™ constituent strains were detected in neonatal LacFi™-administered Thoroughbred feces using polymerase chain reaction denaturing gradient gel electrophoresis and culture methods. These fve strains were found to be the predominant lactobacilli and bifidobacteria in the intestinal microbiota of LacFi™-administered Thoroughbreds. Administration of LacFi™ to neonatal Thoroughbreds decreased diarrhea incidence from 75.9% in the control group (n=29 neonatal Thoroughbreds) to 30.7% in the LacFi™-administered group (n=101 neonatal Thoroughbreds) immediately after birth to 20 weeks after birth. LacFi™ treatment also prevented diarrhea especially at and around 4 weeks and from 10 to 16 weeks. The duration of diarrhea was also shorter in the probiotics-administered group (7.4 ± 0.8 days) than in the control group (14.0 ± 3.2 days). These results indicate that the LacFi™ probiotics regulates intestinal function and contributes to diarrhea prevention.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalJournal of Equine Science
Volume25
Issue number2
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Bifidobacterium
Probiotics
Lactobacillus
probiotics
Diarrhea
diarrhea
Anti-Inflammatory Agents
Parturition
Lactobacillus equi
Bifidobacterium boum
Lactobacillus ruminis
Denaturing Gradient Gel Electrophoresis
Control Groups
Caco-2 Cells
Interleukin-17
Transforming Growth Factors
Lactobacillus johnsonii
Feces
Horses
Lactobacillus reuteri

Keywords

  • Diarrhea prevention
  • Interleukin-17
  • Probiotics
  • Thoroughbred

ASJC Scopus subject areas

  • Equine

Cite this

Anti-inflammatory and intestinal barrier-protective activities of commensal lactobacilli and bifidobacteria in thoroughbreds : Role of probiotics in diarrhea prevention in neonatal Thoroughbreds. / Tanabe, Soichi; Suzuki, Takuya; Wasano, Yuichiro; Nakajima, Fumihiko; Kawasaki, Hiroshi; Tsuda, Tomonori; Nagamine, Natsuko; Tsurumachi, Takashi; Sugaya, Kiyoshi; Akita, Hiroaki; Takagi, Misako; Takagi, Kunihiko; Inoue, Yoshinobu; Asai, Yo; Morita, Hidetoshi.

In: Journal of Equine Science, Vol. 25, No. 2, 2014, p. 37-43.

Research output: Contribution to journalArticle

Tanabe, S, Suzuki, T, Wasano, Y, Nakajima, F, Kawasaki, H, Tsuda, T, Nagamine, N, Tsurumachi, T, Sugaya, K, Akita, H, Takagi, M, Takagi, K, Inoue, Y, Asai, Y & Morita, H 2014, 'Anti-inflammatory and intestinal barrier-protective activities of commensal lactobacilli and bifidobacteria in thoroughbreds: Role of probiotics in diarrhea prevention in neonatal Thoroughbreds', Journal of Equine Science, vol. 25, no. 2, pp. 37-43. https://doi.org/10.1294/jes.25.37
Tanabe, Soichi ; Suzuki, Takuya ; Wasano, Yuichiro ; Nakajima, Fumihiko ; Kawasaki, Hiroshi ; Tsuda, Tomonori ; Nagamine, Natsuko ; Tsurumachi, Takashi ; Sugaya, Kiyoshi ; Akita, Hiroaki ; Takagi, Misako ; Takagi, Kunihiko ; Inoue, Yoshinobu ; Asai, Yo ; Morita, Hidetoshi. / Anti-inflammatory and intestinal barrier-protective activities of commensal lactobacilli and bifidobacteria in thoroughbreds : Role of probiotics in diarrhea prevention in neonatal Thoroughbreds. In: Journal of Equine Science. 2014 ; Vol. 25, No. 2. pp. 37-43.
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