Anti-HMGB1 antibody reduces weight gain in mice fed a high-fat diet

V. N. Montes, S. Subramanian, L. Goodspeed, S. A. Wang, M. Omer, A. Bobik, Kiyoshi Teshigawara, Masahiro Nishibori, A. Chait

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Insulin resistance in obesity is believed to be propagated by adipose tissue and liver inflammation. HMGB1 is a multifunctional protein that is pro-inflammatory when released from cells. It has been previously demonstrated that anti-HMGB1 antibody reduces atherosclerotic lesion pro-inflammatory cells and progression of atherosclerosis in a mouse model. To test the potential beneficial role of blocking HMGB1 in adipose tissue and liver inflammation in mice fed an obesogenic diet, we administered anti-HMGB1 antibody to C57Bl/6 mice fed a high (60%)-fat diet. The mice were treated with weekly injections of an anti-HMGB1 antibody or anti-KLH antibody (isotype control) for 16 weeks. Mice that received the anti-HMGB1 antibody gained less weight than the control-treated animals. Anti-HMGB1 treatment also reduced hepatic expression of TNF-alpha and MCP-1, molecules that promote inflammation. However, adipose tissue inflammation, as measured by gene expression analyses and immunohistochemistry, did not differ between the two groups. There also were no differences in glucose or insulin tolerance between the two groups. When feeding mice a high-fat diet, these data suggest that HMGB1 may have a crucial role in weight gain and liver inflammation.

Original languageEnglish
Article numbere161
JournalNutrition and Diabetes
Volume5
DOIs
Publication statusPublished - Jun 15 2015

Fingerprint

HMGB1 Protein
High Fat Diet
Weight Gain
Anti-Idiotypic Antibodies
Inflammation
Adipose Tissue
Liver
Insulin Resistance
Atherosclerosis
Tumor Necrosis Factor-alpha
Obesity
Immunohistochemistry
Insulin
Diet
Gene Expression
Weights and Measures
Glucose
Injections

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Montes, V. N., Subramanian, S., Goodspeed, L., Wang, S. A., Omer, M., Bobik, A., ... Chait, A. (2015). Anti-HMGB1 antibody reduces weight gain in mice fed a high-fat diet. Nutrition and Diabetes, 5, [e161]. https://doi.org/10.1038/nutd.2015.11

Anti-HMGB1 antibody reduces weight gain in mice fed a high-fat diet. / Montes, V. N.; Subramanian, S.; Goodspeed, L.; Wang, S. A.; Omer, M.; Bobik, A.; Teshigawara, Kiyoshi; Nishibori, Masahiro; Chait, A.

In: Nutrition and Diabetes, Vol. 5, e161, 15.06.2015.

Research output: Contribution to journalArticle

Montes, VN, Subramanian, S, Goodspeed, L, Wang, SA, Omer, M, Bobik, A, Teshigawara, K, Nishibori, M & Chait, A 2015, 'Anti-HMGB1 antibody reduces weight gain in mice fed a high-fat diet', Nutrition and Diabetes, vol. 5, e161. https://doi.org/10.1038/nutd.2015.11
Montes VN, Subramanian S, Goodspeed L, Wang SA, Omer M, Bobik A et al. Anti-HMGB1 antibody reduces weight gain in mice fed a high-fat diet. Nutrition and Diabetes. 2015 Jun 15;5. e161. https://doi.org/10.1038/nutd.2015.11
Montes, V. N. ; Subramanian, S. ; Goodspeed, L. ; Wang, S. A. ; Omer, M. ; Bobik, A. ; Teshigawara, Kiyoshi ; Nishibori, Masahiro ; Chait, A. / Anti-HMGB1 antibody reduces weight gain in mice fed a high-fat diet. In: Nutrition and Diabetes. 2015 ; Vol. 5.
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