Anti-allergic effects of Vernonia amygdalina leaf extracts in hapten-induced atopic dermatitis-like disease in mice

Nlandu Roger Ngatu, Maiko K. Okajima, Maki Yokogawa, Ryoji Hirota, Mikiro Takaishi, Masamitsu Eitoku, Basilua Andre Muzembo, Asif Bhati Sabah, Takao Saruta, Mitsuhiko Miyamura, Tatsuo Kaneko, Shigetoshi Sano, Narufumi Suganuma

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by pruritic and eczematous skin lesions. In this study, AD-like disease was induced in NC/Nga mice so as to evaluate the antiallergic effects of Vernonia amygdalina leaf extracts (VAM). Methods: Forty NC/Nga mice were purchased for each of the two protocols (prophylactic and curative) of the study. Mice were randomly divided in groups of five or six after sensitization with 5% trinitrochlorobenzene (TNCB): aqueous extracts (VAM1), methanolic extracts (VAM2), hydrocortisone (HCT), buffer for the control (TNCB) and the normal mice (NORM) groups. Results: As for HCT, VAM1 and VAM2-pretreated mice showed significantly lower number of scratching behavior episodes (p < 0.01; vs. TNCB) following TNCB challenge. In addition, VAM1, VAM2 exerted a significant inhibitory effect on the development of AD skin symptoms (vs. TNCB group; p < 0.001), the production of IgE, TNF-alpha (p < 0.05), IL-5 and IFN-gamma (p < 0.01) (vs. TNCB group) and on the increase in ear thickness (p < 0.05) in prophylactic protocol. In the AD curative protocol, topical VAM1, VAM2 markedly improved skin lesions such as erythema/hemorrhage (p < 0.05), scaling/dryness, erosion/excoriation (p < 0.01) (vs. TNCB mice). Furthermore, a significant decrease in ear thickness was noted in VAM1, VAM2, HCT groups (vs. TNCB group; p < 0.05) as well as the serum total IgE, MCP-1 (p < 0.01) and eotaxin (p < 0.05). VAM2 also improved chronic eczema dermatitis skin symptoms in a patient. Conclusions: Results from this report suggest that VAM extracts, known as ERK pathway inhibitor, prevent and improve atopic/eczema dermatitis syndrome.

Original languageEnglish
Pages (from-to)597-607
Number of pages11
JournalAllergology International
Volume61
Issue number4
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Atopic dermatitis
  • Eosinophilia
  • Scratch
  • Vernonia amygdalina

ASJC Scopus subject areas

  • Immunology and Allergy

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