Anterior cruciate ligament-derived cells have high chondrogenic potential

Takayuki Furumatsu, Motomi Hachioji, Kenta Saiga, Naoki Takata, Yusuke Yokoyama, Toshifumi Ozaki

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Anterior cruciate ligament (ACL)-derived cells have a character different from medial collateral ligament (MCL)-derived cells. However, the critical difference between ACL and MCL is still unclear in their healing potential and cellular response. The objective of this study was to investigate the mesenchymal differentiation property of each ligament-derived cell. Both ligament-derived cells differentiated into adipogenic, osteogenic, and chondrogenic lineages. In chondrogenesis, ACL-derived cells had the higher chondrogenic property than MCL-derived cells. The chondrogenic marker genes, Sox9 and α1(II) collagen (Col2a1), were induced faster in ACL-derived pellets than in MCL-derived pellets. Sox9 expression preceded the increase of Col2a1 in both pellet-cultured cells. However, the expression level of Sox9 and a ligament/tendon transcription factor Scleraxis did not parallel the increase of Col2a1 expression along with chondrogenic induction. The present study demonstrates that the balance between Sox9 and Scleraxis have an important role in the chondrogenic differentiation of ligament-derived cells.

Original languageEnglish
Pages (from-to)1142-1147
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume391
Issue number1
DOIs
Publication statusPublished - Jan 1 2010

Keywords

  • Anterior cruciate ligament
  • Chondrogenesis
  • Differentiation
  • Medial collateral ligament
  • Scleraxis
  • Sox9

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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