TY - JOUR
T1 - Anterior cruciate ligament-derived cells have high chondrogenic potential
AU - Furumatsu, Takayuki
AU - Hachioji, Motomi
AU - Saiga, Kenta
AU - Takata, Naoki
AU - Yokoyama, Yusuke
AU - Ozaki, Toshifumi
N1 - Funding Information:
This work was supported by Japan Society for the Promotion of Science (Nos. 18890115 and 20791040 ), the Japanese Foundation for Research and Promotion of Endoscopy, Ryobi Teien Memory Foundation, and Child Health and Development from the Ministry of Health, Labor and Welfare (20-3) (T.F.).
PY - 2010/1/1
Y1 - 2010/1/1
N2 - Anterior cruciate ligament (ACL)-derived cells have a character different from medial collateral ligament (MCL)-derived cells. However, the critical difference between ACL and MCL is still unclear in their healing potential and cellular response. The objective of this study was to investigate the mesenchymal differentiation property of each ligament-derived cell. Both ligament-derived cells differentiated into adipogenic, osteogenic, and chondrogenic lineages. In chondrogenesis, ACL-derived cells had the higher chondrogenic property than MCL-derived cells. The chondrogenic marker genes, Sox9 and α1(II) collagen (Col2a1), were induced faster in ACL-derived pellets than in MCL-derived pellets. Sox9 expression preceded the increase of Col2a1 in both pellet-cultured cells. However, the expression level of Sox9 and a ligament/tendon transcription factor Scleraxis did not parallel the increase of Col2a1 expression along with chondrogenic induction. The present study demonstrates that the balance between Sox9 and Scleraxis have an important role in the chondrogenic differentiation of ligament-derived cells.
AB - Anterior cruciate ligament (ACL)-derived cells have a character different from medial collateral ligament (MCL)-derived cells. However, the critical difference between ACL and MCL is still unclear in their healing potential and cellular response. The objective of this study was to investigate the mesenchymal differentiation property of each ligament-derived cell. Both ligament-derived cells differentiated into adipogenic, osteogenic, and chondrogenic lineages. In chondrogenesis, ACL-derived cells had the higher chondrogenic property than MCL-derived cells. The chondrogenic marker genes, Sox9 and α1(II) collagen (Col2a1), were induced faster in ACL-derived pellets than in MCL-derived pellets. Sox9 expression preceded the increase of Col2a1 in both pellet-cultured cells. However, the expression level of Sox9 and a ligament/tendon transcription factor Scleraxis did not parallel the increase of Col2a1 expression along with chondrogenic induction. The present study demonstrates that the balance between Sox9 and Scleraxis have an important role in the chondrogenic differentiation of ligament-derived cells.
KW - Anterior cruciate ligament
KW - Chondrogenesis
KW - Differentiation
KW - Medial collateral ligament
KW - Scleraxis
KW - Sox9
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U2 - 10.1016/j.bbrc.2009.12.044
DO - 10.1016/j.bbrc.2009.12.044
M3 - Article
C2 - 20006576
AN - SCOPUS:72949119939
SN - 0006-291X
VL - 391
SP - 1142
EP - 1147
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -