Antagonism between SA- and JA-signaling conditioned by saccharin in Arabidopsis thaliana renders resistance to a specific pathogen

Le Thi Phuong, Aprilia Nur Fitrianti, Mai Thanh Luan, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

Research output: Contribution to journalArticle

Abstract

Saccharin is generated from probenazole (PBZ) in plants and acts as a plant defense activator. Our study of the mechanism underlying saccharin-induced systemic acquired resistance in Arabidopsis thaliana suggests an antagonistic interaction between salicylic acid (SA)- and jasmonic acid (JA)-signaling as revealed through gene expression analyses. In wild-type plants (Col-0) exposed to saccharin, there was a consistent increase in callose deposition and in expression of SA-marker genes, PR1 and PR2, which coincided with a decrease in expression of JA-marker genes such as VSP2, LOX2 and PDF1.2. Actually, pretreatment of Col-0 with saccharin or PBZ conferred resistance to Pseudomonas syringae pv. tomato DC3000, but not to Pectobacterium carotovorum subsp. carotovorum, Botrytis cinerea, or Colletotrichum higginsianum. Enhanced expression of SA- and JA-marker genes and the augmented deposition of callose were evident after a challenge with virulent DC3000 in saccharin-pretreated plants. Consistently, pretreatment of saccharin and PBZ with SA- and JA-defective mutants led to diminished resistance in NahG-transgenic and npr1 mutant plants, but not in jar1 mutant plants, suggesting that saccharin and PBZ induce resistance in A. thaliana against Pto DC3000 mainly via activation of SA-signaling, leading to suppression of JA/ET-signaling and vice versa. Collectively, an antagonism between SA- and JA-signaling conditioned by saccharin renders resistance to a specific pathogen in Arabidopsis.

Original languageEnglish
JournalJournal of General Plant Pathology
DOIs
Publication statusAccepted/In press - Jan 1 2019

Fingerprint

saccharin
jasmonic acid
salicylic acid
Arabidopsis thaliana
pathogens
callose
mutants
genetic markers
pretreatment
Pectobacterium carotovorum subsp. carotovorum
Pseudomonas syringae pv. tomato
systemic acquired resistance
Botrytis cinerea
Arabidopsis
genetically modified organisms
gene expression

Keywords

  • Induced resistance
  • Jasmonic acid (JA)
  • Probenazole (PBZ)
  • Saccharin
  • Salicylic acid (SA)
  • Signaling

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Antagonism between SA- and JA-signaling conditioned by saccharin in Arabidopsis thaliana renders resistance to a specific pathogen. / Phuong, Le Thi; Fitrianti, Aprilia Nur; Luan, Mai Thanh; Matsui, Hidenori; Noutoshi, Yoshiteru; Yamamoto, Mikihiro; Ichinose, Yuki; Shiraishi, Tomonori; Toyoda, Kazuhiro.

In: Journal of General Plant Pathology, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Fitrianti, Aprilia Nur

AU - Luan, Mai Thanh

AU - Matsui, Hidenori

AU - Noutoshi, Yoshiteru

AU - Yamamoto, Mikihiro

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AU - Shiraishi, Tomonori

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