TY - JOUR
T1 - Antagonism between SA- and JA-signaling conditioned by saccharin in Arabidopsis thaliana renders resistance to a specific pathogen
AU - Phuong, Le Thi
AU - Fitrianti, Aprilia Nur
AU - Luan, Mai Thanh
AU - Matsui, Hidenori
AU - Noutoshi, Yoshiteru
AU - Yamamoto, Mikihiro
AU - Ichinose, Yuki
AU - Shiraishi, Tomonori
AU - Toyoda, Kazuhiro
N1 - Funding Information:
LTP thanks the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) for their financial support during her doctoral course. We acknowledge Dr. Shinji Tsuyumu (Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan) for generously providing the Pectobacterium carotovorum subsp. carotovorum strain Pc1. This research was supported in part by the Grants-in-Aid for Scientific Research (18K05645) from the Japan Society for Promotion of Science (JSPS).
Funding Information:
LTP thanks the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) for their financial support during her doctoral course. We acknowledge Dr. Shinji Tsuyumu (Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan) for generously providing the Pectobacterium carotovorum subsp. carotovorum strain Pc1. This research was supported in part by the Grants-in-Aid for Scientific Research (18K05645) from the Japan Society for Promotion of Science (JSPS).
Publisher Copyright:
© 2019, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Saccharin is generated from probenazole (PBZ) in plants and acts as a plant defense activator. Our study of the mechanism underlying saccharin-induced systemic acquired resistance in Arabidopsis thaliana suggests an antagonistic interaction between salicylic acid (SA)- and jasmonic acid (JA)-signaling as revealed through gene expression analyses. In wild-type plants (Col-0) exposed to saccharin, there was a consistent increase in callose deposition and in expression of SA-marker genes, PR1 and PR2, which coincided with a decrease in expression of JA-marker genes such as VSP2, LOX2 and PDF1.2. Actually, pretreatment of Col-0 with saccharin or PBZ conferred resistance to Pseudomonas syringae pv. tomato DC3000, but not to Pectobacterium carotovorum subsp. carotovorum, Botrytis cinerea, or Colletotrichum higginsianum. Enhanced expression of SA- and JA-marker genes and the augmented deposition of callose were evident after a challenge with virulent DC3000 in saccharin-pretreated plants. Consistently, pretreatment of saccharin and PBZ with SA- and JA-defective mutants led to diminished resistance in NahG-transgenic and npr1 mutant plants, but not in jar1 mutant plants, suggesting that saccharin and PBZ induce resistance in A. thaliana against Pto DC3000 mainly via activation of SA-signaling, leading to suppression of JA/ET-signaling and vice versa. Collectively, an antagonism between SA- and JA-signaling conditioned by saccharin renders resistance to a specific pathogen in Arabidopsis.
AB - Saccharin is generated from probenazole (PBZ) in plants and acts as a plant defense activator. Our study of the mechanism underlying saccharin-induced systemic acquired resistance in Arabidopsis thaliana suggests an antagonistic interaction between salicylic acid (SA)- and jasmonic acid (JA)-signaling as revealed through gene expression analyses. In wild-type plants (Col-0) exposed to saccharin, there was a consistent increase in callose deposition and in expression of SA-marker genes, PR1 and PR2, which coincided with a decrease in expression of JA-marker genes such as VSP2, LOX2 and PDF1.2. Actually, pretreatment of Col-0 with saccharin or PBZ conferred resistance to Pseudomonas syringae pv. tomato DC3000, but not to Pectobacterium carotovorum subsp. carotovorum, Botrytis cinerea, or Colletotrichum higginsianum. Enhanced expression of SA- and JA-marker genes and the augmented deposition of callose were evident after a challenge with virulent DC3000 in saccharin-pretreated plants. Consistently, pretreatment of saccharin and PBZ with SA- and JA-defective mutants led to diminished resistance in NahG-transgenic and npr1 mutant plants, but not in jar1 mutant plants, suggesting that saccharin and PBZ induce resistance in A. thaliana against Pto DC3000 mainly via activation of SA-signaling, leading to suppression of JA/ET-signaling and vice versa. Collectively, an antagonism between SA- and JA-signaling conditioned by saccharin renders resistance to a specific pathogen in Arabidopsis.
KW - Induced resistance
KW - Jasmonic acid (JA)
KW - Probenazole (PBZ)
KW - Saccharin
KW - Salicylic acid (SA)
KW - Signaling
UR - http://www.scopus.com/inward/record.url?scp=85075698248&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85075698248&partnerID=8YFLogxK
U2 - 10.1007/s10327-019-00899-x
DO - 10.1007/s10327-019-00899-x
M3 - Article
AN - SCOPUS:85075698248
VL - 86
SP - 86
EP - 99
JO - Journal of General Plant Pathology
JF - Journal of General Plant Pathology
SN - 1345-2630
IS - 2
ER -