Ant28 gene for proanthocyanidin synthesis encoding the R2R3 MYB domain protein (Hvmyb10) highly affects grain dormancy in barley

Eiko Himi, Yuko Yamashita, Naoto Haruyama, Takashi Yanagisawa, Masahiko Maekawa, Shin Taketa

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A number of anthocyanin- and proanthocyanidin-free mutants (ant mutants) in barley were induced and selected because of breeding interest to reduce proanthocyanidins, which could cause haze and degrade the quality of beer. Ant loci, known as anthocyanin or proanthocyanidin synthesis genes, are classified into Ant1 to Ant30 through allelism tests. However, only the Ant18 gene has been molecularly shown to encode dihydroflavonol 4-reductase (DFR), which is involved in both anthocyanin and proanthocyanidin synthesis. In this study, an R2R3 MYB gene of barley was isolated by PCR and named Hvmyb10 due to its similarity to Tamyb10 of wheat, which is a candidate for the R-1 gene grain color regulator. The predicted amino acid sequences of Hvmyb10 showed high similarity not only to Tamyb10 but also to TT2, the proanthocyanidin regulator of Arabidopsis. Non-synonymous nucleotide substitutions in the Hvmyb10 gene were found in all six ant28 mutants tested. Mapping showed that a polymorphism in Hvmyb10 perfectly cosegregated with the ant 28 phenotype on the distal region of the long arm of chromosome 3H. These results demonstrate that ant28 encodes Hvmyb10, the R2R3 MYB domain protein that regulates proanthocyanidin accumulation in developing grains. The reduced grain dormancy of ant28 mutants compared with those of the respective wild types indicates that Hvmyb10 is a key factor in grain dormancy in barley.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalEuphytica
Volume188
Issue number1
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Hordeum
proanthocyanidins
dormancy
barley
Anthocyanins
synthesis
Ants
Genes
anthocyanins
genes
proteins
mutants
Genetic Complementation Test
Formicidae
vpr Genes
Proanthocyanidins
allelism
Arabidopsis
Triticum
Breeding

Keywords

  • Ant mutants
  • Barley
  • Grain dormancy
  • Proanthocyanidin

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Horticulture
  • Plant Science

Cite this

Ant28 gene for proanthocyanidin synthesis encoding the R2R3 MYB domain protein (Hvmyb10) highly affects grain dormancy in barley. / Himi, Eiko; Yamashita, Yuko; Haruyama, Naoto; Yanagisawa, Takashi; Maekawa, Masahiko; Taketa, Shin.

In: Euphytica, Vol. 188, No. 1, 11.2012, p. 141-151.

Research output: Contribution to journalArticle

Himi, Eiko ; Yamashita, Yuko ; Haruyama, Naoto ; Yanagisawa, Takashi ; Maekawa, Masahiko ; Taketa, Shin. / Ant28 gene for proanthocyanidin synthesis encoding the R2R3 MYB domain protein (Hvmyb10) highly affects grain dormancy in barley. In: Euphytica. 2012 ; Vol. 188, No. 1. pp. 141-151.
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