Annexin V Assay-proven Anti-apoptotic Effect of Ascorbic Acid 2-glucoside after Cold Ischemia/Reperfusion Injury in Rat Liver Transplantation

Jie Liu, Takahito Yagi, Hiroshi Sadamori, Hiroyoshi Matsukawa, Dong Sheng Sun, Naoshi Mitsuoka, Masao Yamamura, Junji Matsuoka, Zaishun Jin, Itaru Yamamoto, Noriaki Tanaka

Research output: Contribution to journalArticle

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Abstract

Controversy exists over whether the predominant cell death of hepatocytes is due to apoptosis or necrosis after ischemia/reperfusion injury. In this study we investigated the predominant cell death of hepatocytes after cold ischemia/reperfusion injury using the Annexin V-based assay, and evaluated the anti-apoptotic effect of ascorbic acid 2-glucoside (AA-2G) added to the University of Wisconsin solution (UW solution) in rat liver transplantation. The retrieved liver was preserved in 4°C UW solution for 24 h, and then transplanted orthotopically to the syngeneic Wistar recipient. The animals were divided into 2 groups, a control group (n = 10), in which liver grafts were preserved in UW solution (4°C), and an AA-2G group (n = 10), in which liver grafts were preserved in UW solution (4°C) with AA-2G (100 ug/ml). The serum AST level 4 h after reperfusion in the control group was significantly suppressed in the AA-2G group, and the bile production of the liver graft in the AA-2G group was well recovered. The mean survival time in the AA-2G group was significantly improved compared with that in the control group. Annexin-V and Propidium iodide staining 4 h after reperfusion showed a significantly higher percentage of viable hepatocytes in the AA-2G group compared with the control group (93.4 ± 2.0 vs. 80.3 ± 2.1%, P<0.05). In the control group, the main cell death of hepatocytes was apoptosis (early apoptosis: 10.0 ± 4.7%, late apoptosis: 6.4 ± 1.7%). The addition of AA-2G to the UW solution significantly inhibited both early and late apoptotic cell death 4 h after reperfusion (early apoptosis: 0.98 ± 0.88%, late apoptosis: 2.2 ± 1.1%). The expression of caspase 9 in the immunostaining of the liver graft was suppressed in the AA-2G group compared with in the control group. Our study using the Annexin V-based assay provided evidence that the predominant cell death of hepatocytes was apoptosis after 24 h cold ischemia/reperfusion injury in rat liver transplantation. The addition of AA-2G to the UW solution attenuated 24 h cold ischemia/reperfusion injury by inhibiting the apoptosis of hepatocytes.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalActa Medica Okayama
Volume57
Issue number5
Publication statusPublished - Oct 2003

Fingerprint

Cold Ischemia
Annexin A5
Reperfusion Injury
Liver
Liver Transplantation
Rats
Assays
Apoptosis
Cell death
Hepatocytes
Cell Death
Grafts
Control Groups
Reperfusion
Transplants
ascorbic acid 2-O-glucoside
Caspase 9
Propidium
Bile
Animals

Keywords

  • Apoptosis
  • Ascorbic acid 2-glucoside (AA-2G)
  • Ischemia/reperfusion injury
  • Liver transplantation

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Annexin V Assay-proven Anti-apoptotic Effect of Ascorbic Acid 2-glucoside after Cold Ischemia/Reperfusion Injury in Rat Liver Transplantation. / Liu, Jie; Yagi, Takahito; Sadamori, Hiroshi; Matsukawa, Hiroyoshi; Sun, Dong Sheng; Mitsuoka, Naoshi; Yamamura, Masao; Matsuoka, Junji; Jin, Zaishun; Yamamoto, Itaru; Tanaka, Noriaki.

In: Acta Medica Okayama, Vol. 57, No. 5, 10.2003, p. 209-216.

Research output: Contribution to journalArticle

Liu, J, Yagi, T, Sadamori, H, Matsukawa, H, Sun, DS, Mitsuoka, N, Yamamura, M, Matsuoka, J, Jin, Z, Yamamoto, I & Tanaka, N 2003, 'Annexin V Assay-proven Anti-apoptotic Effect of Ascorbic Acid 2-glucoside after Cold Ischemia/Reperfusion Injury in Rat Liver Transplantation', Acta Medica Okayama, vol. 57, no. 5, pp. 209-216.
Liu, Jie ; Yagi, Takahito ; Sadamori, Hiroshi ; Matsukawa, Hiroyoshi ; Sun, Dong Sheng ; Mitsuoka, Naoshi ; Yamamura, Masao ; Matsuoka, Junji ; Jin, Zaishun ; Yamamoto, Itaru ; Tanaka, Noriaki. / Annexin V Assay-proven Anti-apoptotic Effect of Ascorbic Acid 2-glucoside after Cold Ischemia/Reperfusion Injury in Rat Liver Transplantation. In: Acta Medica Okayama. 2003 ; Vol. 57, No. 5. pp. 209-216.
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AU - Matsukawa, Hiroyoshi

AU - Sun, Dong Sheng

AU - Mitsuoka, Naoshi

AU - Yamamura, Masao

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