Annexin A2 regulates angiogenesis and invasion phenotypes of malignant glioma

Manabu Onishi, Tomotsugu Ichikawa, Kazuhiko Kurozumi, Satoshi Inoue, Tomoko Maruo, Yoshihiro Otani, Kentaro Fujii, Joji Ishida, Yosuke Shimazu, Koichi Yoshida, Hiroyuki Michiue, E. Antonio Chiocca, Isao Date

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have established a pair of animal models (J3T-1 and J3T-2) with different invasive and angiogenic phenotypes, and demonstrated that annexin A2 is expressed at higher levels in J3T-1 than J3T-2 cells. The function of annexin A2 in relation to angiogenesis and invasion was investigated using these models. Stable silencing or overexpression of annexin A2 in J3T-1 and J3T-2 cells (J3T-1shA and J3T-2A cells) was established and used. Thirty human glioblastoma samples were evaluated for expression of annexin A2, vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF). Immunohistochemical and quantitative reverse-transcription polymerase chain reaction analyses revealed higher expression of annexin A2, VEGF and PDGF in J3T-1 and J3T-2A cells. Cultured J3T-1 and J3T-2A cells exhibited higher adhesive ability to endothelial cells. Histopathological analysis of animal brain tumors revealed that J3T-1 and J3T-2A tumors displayed marked angiogenesis and invasion along the neovasculature, whereas J3T-2 and J3T-1shA tumors exhibited diffuse, infiltrative invasion without angiogenesis. Positive expression of annexin A2 was observed in tumor cells surrounding dilated vessels in 25/30 human glioblastoma specimens. Our results reveal that the phenotype of glioma invasion is closely related to angiogenesis. We identify annexin A2 as a factor regulating angiogenesis and invasion of malignant gliomas.

Original languageEnglish
Pages (from-to)184-194
Number of pages11
JournalBrain Tumor Pathology
Volume32
Issue number3
DOIs
Publication statusPublished - Feb 20 2015

Fingerprint

Annexin A2
Glioma
Phenotype
Platelet-Derived Growth Factor
Glioblastoma
Vascular Endothelial Growth Factor A
Neoplasms
Angiogenesis Inducing Agents
Brain Neoplasms
Adhesives
Reverse Transcription
Endothelial Cells
Animal Models
Polymerase Chain Reaction

Keywords

  • Angiogenesis
  • Animal model
  • Annexin A2
  • Glioma
  • Invasion

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Clinical Neurology

Cite this

Annexin A2 regulates angiogenesis and invasion phenotypes of malignant glioma. / Onishi, Manabu; Ichikawa, Tomotsugu; Kurozumi, Kazuhiko; Inoue, Satoshi; Maruo, Tomoko; Otani, Yoshihiro; Fujii, Kentaro; Ishida, Joji; Shimazu, Yosuke; Yoshida, Koichi; Michiue, Hiroyuki; Antonio Chiocca, E.; Date, Isao.

In: Brain Tumor Pathology, Vol. 32, No. 3, 20.02.2015, p. 184-194.

Research output: Contribution to journalArticle

Onishi, M, Ichikawa, T, Kurozumi, K, Inoue, S, Maruo, T, Otani, Y, Fujii, K, Ishida, J, Shimazu, Y, Yoshida, K, Michiue, H, Antonio Chiocca, E & Date, I 2015, 'Annexin A2 regulates angiogenesis and invasion phenotypes of malignant glioma', Brain Tumor Pathology, vol. 32, no. 3, pp. 184-194. https://doi.org/10.1007/s10014-015-0216-6
Onishi, Manabu ; Ichikawa, Tomotsugu ; Kurozumi, Kazuhiko ; Inoue, Satoshi ; Maruo, Tomoko ; Otani, Yoshihiro ; Fujii, Kentaro ; Ishida, Joji ; Shimazu, Yosuke ; Yoshida, Koichi ; Michiue, Hiroyuki ; Antonio Chiocca, E. ; Date, Isao. / Annexin A2 regulates angiogenesis and invasion phenotypes of malignant glioma. In: Brain Tumor Pathology. 2015 ; Vol. 32, No. 3. pp. 184-194.
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