Annexin 5 decreases the diffusion of lipid and channel molecules in an artificial lipid bilayer

Takehiko Ichikawa, Yuko Takeuchi, Takaaki Aoki, Toru Ide

Research output: Contribution to journalArticle

Abstract

The effects of annexin 5 on the lateral diffusion of single molecule lipids and single molecule proteins were studied in a lipid bilayer membrane. Single β-BODIPY HPC and ryanodine receptor type 2 (RyR2) labeled with Cy5 molecules were monitored by fluorescence microscopy. The diffusion coefficient was calculated in the presence and absence of annexin 5. The diffusion coefficients of β-BODIPY HPC and RyR2 were 11 × 11 × 10 -8 cm2/s and 2.7 × 10-8 cm2/s in the absence of annexin 5, respectively. The diffusion coefficients of β-BODIPY HPC and RyR2 in the presence of 1 μM annexin 5 were 2.4 × 10-10 cm2/s and 2.6 × 10-10 cm 2/s, respectively. Overall, 1 μM annexin 5 decreases the lateral diffusion coefficient 100-500 fold. Regarding RyR2, annexin 5 has little effect on function and can be used to immobilize RyR2 in a lipid bilayer system.

Original languageEnglish
Journale-Journal of Surface Science and Nanotechnology
Volume3
DOIs
Publication statusPublished - Jun 18 2005
Externally publishedYes

Fingerprint

Annexins
Lipid bilayers
Lipid Bilayers
Ryanodine Receptor Calcium Release Channel
Lipids
lipids
diffusion coefficient
Molecules
molecules
Fluorescence microscopy
Membrane Lipids
Fluorescence Microscopy
membranes
microscopy
proteins
Proteins
Membranes
fluorescence
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

Keywords

  • Biological molecules - proteins
  • Biophysics, medical physics, biomedical engineering
  • Ion channel
  • Nano-scale imaging, measurement, and manipulation technology

ASJC Scopus subject areas

  • Materials Science (miscellaneous)
  • Surfaces, Coatings and Films

Cite this

Annexin 5 decreases the diffusion of lipid and channel molecules in an artificial lipid bilayer. / Ichikawa, Takehiko; Takeuchi, Yuko; Aoki, Takaaki; Ide, Toru.

In: e-Journal of Surface Science and Nanotechnology, Vol. 3, 18.06.2005.

Research output: Contribution to journalArticle

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