Annexin 5 decreases the diffusion of lipid and channel molecules in an artificial lipid bilayer

Takehiko Ichikawa; Yuko Takeuchi; Takaaki Aoki; Toru Ide

doi:10.1380/ejssnt.2005.213

Annexin 5 decreases the diffusion of lipid and channel molecules in an artificial lipid bilayer

Takehiko Ichikawa, Yuko Takeuchi, Takaaki Aoki, Toru Ide

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of annexin 5 on the lateral diffusion of single molecule lipids and single molecule proteins were studied in a lipid bilayer membrane. Single β-BODIPY HPC and ryanodine receptor type 2 (RyR2) labeled with Cy5 molecules were monitored by fluorescence microscopy. The diffusion coefficient was calculated in the presence and absence of annexin 5. The diffusion coefficients of β-BODIPY HPC and RyR2 were 11 × 11 × 10 ^-8 cm²/s and 2.7 × 10^-8 cm²/s in the absence of annexin 5, respectively. The diffusion coefficients of β-BODIPY HPC and RyR2 in the presence of 1 μM annexin 5 were 2.4 × 10^-10 cm²/s and 2.6 × 10^-10 cm ²/s, respectively. Overall, 1 μM annexin 5 decreases the lateral diffusion coefficient 100-500 fold. Regarding RyR2, annexin 5 has little effect on function and can be used to immobilize RyR2 in a lipid bilayer system.

Original language	English
Journal	e-Journal of Surface Science and Nanotechnology
Volume	3
DOIs	https://doi.org/10.1380/ejssnt.2005.213
Publication status	Published - Jun 18 2005
Externally published	Yes

Keywords

Biological molecules - proteins
Biophysics, medical physics, biomedical engineering
Ion channel
Nano-scale imaging, measurement, and manipulation technology

ASJC Scopus subject areas

Biotechnology
Bioengineering
Condensed Matter Physics
Mechanics of Materials
Surfaces and Interfaces
Surfaces, Coatings and Films

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10.1380/ejssnt.2005.213

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title = "Annexin 5 decreases the diffusion of lipid and channel molecules in an artificial lipid bilayer",

abstract = "The effects of annexin 5 on the lateral diffusion of single molecule lipids and single molecule proteins were studied in a lipid bilayer membrane. Single β-BODIPY HPC and ryanodine receptor type 2 (RyR2) labeled with Cy5 molecules were monitored by fluorescence microscopy. The diffusion coefficient was calculated in the presence and absence of annexin 5. The diffusion coefficients of β-BODIPY HPC and RyR2 were 11 × 11 × 10 -8 cm2/s and 2.7 × 10-8 cm2/s in the absence of annexin 5, respectively. The diffusion coefficients of β-BODIPY HPC and RyR2 in the presence of 1 μM annexin 5 were 2.4 × 10-10 cm2/s and 2.6 × 10-10 cm 2/s, respectively. Overall, 1 μM annexin 5 decreases the lateral diffusion coefficient 100-500 fold. Regarding RyR2, annexin 5 has little effect on function and can be used to immobilize RyR2 in a lipid bilayer system.",

keywords = "Biological molecules - proteins, Biophysics, medical physics, biomedical engineering, Ion channel, Nano-scale imaging, measurement, and manipulation technology",

author = "Takehiko Ichikawa and Yuko Takeuchi and Takaaki Aoki and Toru Ide",

year = "2005",

month = jun,

day = "18",

doi = "10.1380/ejssnt.2005.213",

language = "English",

volume = "3",

journal = "e-Journal of Surface Science and Nanotechnology",

issn = "1348-0391",

publisher = "Surface Science Society of Japan",

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TY - JOUR

T1 - Annexin 5 decreases the diffusion of lipid and channel molecules in an artificial lipid bilayer

AU - Ichikawa, Takehiko

AU - Takeuchi, Yuko

AU - Aoki, Takaaki

AU - Ide, Toru

PY - 2005/6/18

Y1 - 2005/6/18

N2 - The effects of annexin 5 on the lateral diffusion of single molecule lipids and single molecule proteins were studied in a lipid bilayer membrane. Single β-BODIPY HPC and ryanodine receptor type 2 (RyR2) labeled with Cy5 molecules were monitored by fluorescence microscopy. The diffusion coefficient was calculated in the presence and absence of annexin 5. The diffusion coefficients of β-BODIPY HPC and RyR2 were 11 × 11 × 10 -8 cm2/s and 2.7 × 10-8 cm2/s in the absence of annexin 5, respectively. The diffusion coefficients of β-BODIPY HPC and RyR2 in the presence of 1 μM annexin 5 were 2.4 × 10-10 cm2/s and 2.6 × 10-10 cm 2/s, respectively. Overall, 1 μM annexin 5 decreases the lateral diffusion coefficient 100-500 fold. Regarding RyR2, annexin 5 has little effect on function and can be used to immobilize RyR2 in a lipid bilayer system.

AB - The effects of annexin 5 on the lateral diffusion of single molecule lipids and single molecule proteins were studied in a lipid bilayer membrane. Single β-BODIPY HPC and ryanodine receptor type 2 (RyR2) labeled with Cy5 molecules were monitored by fluorescence microscopy. The diffusion coefficient was calculated in the presence and absence of annexin 5. The diffusion coefficients of β-BODIPY HPC and RyR2 were 11 × 11 × 10 -8 cm2/s and 2.7 × 10-8 cm2/s in the absence of annexin 5, respectively. The diffusion coefficients of β-BODIPY HPC and RyR2 in the presence of 1 μM annexin 5 were 2.4 × 10-10 cm2/s and 2.6 × 10-10 cm 2/s, respectively. Overall, 1 μM annexin 5 decreases the lateral diffusion coefficient 100-500 fold. Regarding RyR2, annexin 5 has little effect on function and can be used to immobilize RyR2 in a lipid bilayer system.

KW - Biological molecules - proteins

KW - Biophysics, medical physics, biomedical engineering

KW - Ion channel

KW - Nano-scale imaging, measurement, and manipulation technology

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DO - 10.1380/ejssnt.2005.213

M3 - Article

AN - SCOPUS:21344466717

SN - 1348-0391

VL - 3

JO - e-Journal of Surface Science and Nanotechnology

JF - e-Journal of Surface Science and Nanotechnology

ER -

Annexin 5 decreases the diffusion of lipid and channel molecules in an artificial lipid bilayer

Abstract

Keywords

ASJC Scopus subject areas

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