Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation

Takanori Tsuji, Yeqing Sun, Koji Kishimoto, Karen A. Olson, Shumei Liu, Saori Hirukawa, Guo Fu Hu

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Angiogenin is an angiogenic protein known to play a role in rRNA transcription in endothelial cells. Nuclear translocation of angiogenin in endothelial cells decreases as cell density increases and ceases when cells are confluent. Here we report that angiogenin is constantly translocated to the nucleus of HeLa cells in a cell density-independent manner. Down-regulation of angiogenin expression by antisense and RNA interference results in a decrease in rRNA transcription, ribosome biogenesis, proliferation, and tumorigenesis both in vitro and in vivo. Exogenous angiogenin rescues the cells from antisense and RNA interference inhibition. The results showed that angiogenin is constitutively translocated into the nucleus of HeLa cells where it stimulates rRNA transcription. Thus, besides its angiogenic activity, angiogenin also plays a role in cancer cell proliferation.

Original languageEnglish
Pages (from-to)1352-1360
Number of pages9
JournalCancer Research
Volume65
Issue number4
DOIs
Publication statusPublished - Feb 15 2005

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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