Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation

Takanori Tsuji, Yeqing Sun, Koji Kishimoto, Karen A. Olson, Shumei Liu, Saori Hirukawa, Guo Fu Hu

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Angiogenin is an angiogenic protein known to play a role in rRNA transcription in endothelial cells. Nuclear translocation of angiogenin in endothelial cells decreases as cell density increases and ceases when cells are confluent. Here we report that angiogenin is constantly translocated to the nucleus of HeLa cells in a cell density-independent manner. Down-regulation of angiogenin expression by antisense and RNA interference results in a decrease in rRNA transcription, ribosome biogenesis, proliferation, and tumorigenesis both in vitro and in vivo. Exogenous angiogenin rescues the cells from antisense and RNA interference inhibition. The results showed that angiogenin is constitutively translocated into the nucleus of HeLa cells where it stimulates rRNA transcription. Thus, besides its angiogenic activity, angiogenin also plays a role in cancer cell proliferation.

Original languageEnglish
Pages (from-to)1352-1360
Number of pages9
JournalCancer Research
Volume65
Issue number4
DOIs
Publication statusPublished - Feb 15 2005

Fingerprint

Ribosomal RNA
HeLa Cells
Cell Proliferation
Antisense RNA
RNA Interference
Endothelial Cells
Cell Count
Angiogenic Proteins
angiogenin
Ribosomes
Carcinogenesis
Down-Regulation
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation. / Tsuji, Takanori; Sun, Yeqing; Kishimoto, Koji; Olson, Karen A.; Liu, Shumei; Hirukawa, Saori; Hu, Guo Fu.

In: Cancer Research, Vol. 65, No. 4, 15.02.2005, p. 1352-1360.

Research output: Contribution to journalArticle

Tsuji, Takanori ; Sun, Yeqing ; Kishimoto, Koji ; Olson, Karen A. ; Liu, Shumei ; Hirukawa, Saori ; Hu, Guo Fu. / Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation. In: Cancer Research. 2005 ; Vol. 65, No. 4. pp. 1352-1360.
@article{6d95a6cc3fe145e2964d3d8420dd175d,
title = "Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation",
abstract = "Angiogenin is an angiogenic protein known to play a role in rRNA transcription in endothelial cells. Nuclear translocation of angiogenin in endothelial cells decreases as cell density increases and ceases when cells are confluent. Here we report that angiogenin is constantly translocated to the nucleus of HeLa cells in a cell density-independent manner. Down-regulation of angiogenin expression by antisense and RNA interference results in a decrease in rRNA transcription, ribosome biogenesis, proliferation, and tumorigenesis both in vitro and in vivo. Exogenous angiogenin rescues the cells from antisense and RNA interference inhibition. The results showed that angiogenin is constitutively translocated into the nucleus of HeLa cells where it stimulates rRNA transcription. Thus, besides its angiogenic activity, angiogenin also plays a role in cancer cell proliferation.",
author = "Takanori Tsuji and Yeqing Sun and Koji Kishimoto and Olson, {Karen A.} and Shumei Liu and Saori Hirukawa and Hu, {Guo Fu}",
year = "2005",
month = "2",
day = "15",
doi = "10.1158/0008-5472.CAN-04-2058",
language = "English",
volume = "65",
pages = "1352--1360",
journal = "Journal of Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "4",

}

TY - JOUR

T1 - Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation

AU - Tsuji, Takanori

AU - Sun, Yeqing

AU - Kishimoto, Koji

AU - Olson, Karen A.

AU - Liu, Shumei

AU - Hirukawa, Saori

AU - Hu, Guo Fu

PY - 2005/2/15

Y1 - 2005/2/15

N2 - Angiogenin is an angiogenic protein known to play a role in rRNA transcription in endothelial cells. Nuclear translocation of angiogenin in endothelial cells decreases as cell density increases and ceases when cells are confluent. Here we report that angiogenin is constantly translocated to the nucleus of HeLa cells in a cell density-independent manner. Down-regulation of angiogenin expression by antisense and RNA interference results in a decrease in rRNA transcription, ribosome biogenesis, proliferation, and tumorigenesis both in vitro and in vivo. Exogenous angiogenin rescues the cells from antisense and RNA interference inhibition. The results showed that angiogenin is constitutively translocated into the nucleus of HeLa cells where it stimulates rRNA transcription. Thus, besides its angiogenic activity, angiogenin also plays a role in cancer cell proliferation.

AB - Angiogenin is an angiogenic protein known to play a role in rRNA transcription in endothelial cells. Nuclear translocation of angiogenin in endothelial cells decreases as cell density increases and ceases when cells are confluent. Here we report that angiogenin is constantly translocated to the nucleus of HeLa cells in a cell density-independent manner. Down-regulation of angiogenin expression by antisense and RNA interference results in a decrease in rRNA transcription, ribosome biogenesis, proliferation, and tumorigenesis both in vitro and in vivo. Exogenous angiogenin rescues the cells from antisense and RNA interference inhibition. The results showed that angiogenin is constitutively translocated into the nucleus of HeLa cells where it stimulates rRNA transcription. Thus, besides its angiogenic activity, angiogenin also plays a role in cancer cell proliferation.

UR - http://www.scopus.com/inward/record.url?scp=13944277097&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13944277097&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-04-2058

DO - 10.1158/0008-5472.CAN-04-2058

M3 - Article

C2 - 15735021

AN - SCOPUS:13944277097

VL - 65

SP - 1352

EP - 1360

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0008-5472

IS - 4

ER -