Angiogenesis Inhibitor TNP-470 Can Suppress Hepatocellular Carcinoma Growth Without Retarding Liver Regeneration after Partial Hepatectomy

Shigeki Kinoshita, Ryuji Hirai, Toshihisa Yamano, Ichiro Yuasa, Kazunori Tsukuda, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Purpose. We investigated the suppressive effect of the angiogenesis inhibitor TNP-470 on accelerated hepatocellular carcinoma (HCC) growth in the regenerating liver. Methods. After 70% partial hepatectomy (PH), AH-130 cells were injected into the portal vein of Donryu rats. A control group was given the vehicle only, and the treated group was given 10mg/kg TNP-470 subcutaneously every second day, from 24 h after tumor implantation, seven times. On day 14, tumor growth was evaluated by the number of foci on the liver surface, liver weight, and the microvessel density of the tumor. Results. The number of foci was significantly less in the treated group (116.5 ± 103.1) than in the control group (319.3 ± 223.1) (P <0.05), as was microvessel density, which was 31.3 ± 14.0/mm2 in the treated group and 61.2 ± 18.9/mm2 in the control group (P <0.05). The liver tended to weigh less in the treated group (12.15 ± 1.28 g) than in the control group (15.22 ± 5.35 g). We also assessed whether TNP-470 retards liver regeneration. Seven days after 70% PH, the liver weight in the treated group was similar to that in the control group. Total bilirubin, serum glutamic oxaloacetic transaminase, and serum glutamic pyruvic transaminase were not higher in the treated group than in the control group. Conclusion. TNP-470 can suppress HCC growth without retarding liver regeneration after PH.

Original languageEnglish
Pages (from-to)40-46
Number of pages7
JournalSurgery Today
Volume34
Issue number1
DOIs
Publication statusPublished - 2004

Fingerprint

Liver Regeneration
Angiogenesis Inhibitors
Hepatectomy
Hepatocellular Carcinoma
Control Groups
Growth
Liver
Microvessels
Weights and Measures
Neoplasms
Aspartate Aminotransferases
Portal Vein
Alanine Transaminase
Bilirubin
O-(chloroacetylcarbamoyl)fumagillol
Serum

Keywords

  • Angiogenesis inhibitor
  • Hepatocellular carcinoma
  • Liver regeneration
  • Partial hepatectomy
  • TNP-470

ASJC Scopus subject areas

  • Surgery

Cite this

Angiogenesis Inhibitor TNP-470 Can Suppress Hepatocellular Carcinoma Growth Without Retarding Liver Regeneration after Partial Hepatectomy. / Kinoshita, Shigeki; Hirai, Ryuji; Yamano, Toshihisa; Yuasa, Ichiro; Tsukuda, Kazunori; Shimizu, Nobuyoshi.

In: Surgery Today, Vol. 34, No. 1, 2004, p. 40-46.

Research output: Contribution to journalArticle

Kinoshita, Shigeki ; Hirai, Ryuji ; Yamano, Toshihisa ; Yuasa, Ichiro ; Tsukuda, Kazunori ; Shimizu, Nobuyoshi. / Angiogenesis Inhibitor TNP-470 Can Suppress Hepatocellular Carcinoma Growth Without Retarding Liver Regeneration after Partial Hepatectomy. In: Surgery Today. 2004 ; Vol. 34, No. 1. pp. 40-46.
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abstract = "Purpose. We investigated the suppressive effect of the angiogenesis inhibitor TNP-470 on accelerated hepatocellular carcinoma (HCC) growth in the regenerating liver. Methods. After 70{\%} partial hepatectomy (PH), AH-130 cells were injected into the portal vein of Donryu rats. A control group was given the vehicle only, and the treated group was given 10mg/kg TNP-470 subcutaneously every second day, from 24 h after tumor implantation, seven times. On day 14, tumor growth was evaluated by the number of foci on the liver surface, liver weight, and the microvessel density of the tumor. Results. The number of foci was significantly less in the treated group (116.5 ± 103.1) than in the control group (319.3 ± 223.1) (P <0.05), as was microvessel density, which was 31.3 ± 14.0/mm2 in the treated group and 61.2 ± 18.9/mm2 in the control group (P <0.05). The liver tended to weigh less in the treated group (12.15 ± 1.28 g) than in the control group (15.22 ± 5.35 g). We also assessed whether TNP-470 retards liver regeneration. Seven days after 70{\%} PH, the liver weight in the treated group was similar to that in the control group. Total bilirubin, serum glutamic oxaloacetic transaminase, and serum glutamic pyruvic transaminase were not higher in the treated group than in the control group. Conclusion. TNP-470 can suppress HCC growth without retarding liver regeneration after PH.",
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AU - Yuasa, Ichiro

AU - Tsukuda, Kazunori

AU - Shimizu, Nobuyoshi

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N2 - Purpose. We investigated the suppressive effect of the angiogenesis inhibitor TNP-470 on accelerated hepatocellular carcinoma (HCC) growth in the regenerating liver. Methods. After 70% partial hepatectomy (PH), AH-130 cells were injected into the portal vein of Donryu rats. A control group was given the vehicle only, and the treated group was given 10mg/kg TNP-470 subcutaneously every second day, from 24 h after tumor implantation, seven times. On day 14, tumor growth was evaluated by the number of foci on the liver surface, liver weight, and the microvessel density of the tumor. Results. The number of foci was significantly less in the treated group (116.5 ± 103.1) than in the control group (319.3 ± 223.1) (P <0.05), as was microvessel density, which was 31.3 ± 14.0/mm2 in the treated group and 61.2 ± 18.9/mm2 in the control group (P <0.05). The liver tended to weigh less in the treated group (12.15 ± 1.28 g) than in the control group (15.22 ± 5.35 g). We also assessed whether TNP-470 retards liver regeneration. Seven days after 70% PH, the liver weight in the treated group was similar to that in the control group. Total bilirubin, serum glutamic oxaloacetic transaminase, and serum glutamic pyruvic transaminase were not higher in the treated group than in the control group. Conclusion. TNP-470 can suppress HCC growth without retarding liver regeneration after PH.

AB - Purpose. We investigated the suppressive effect of the angiogenesis inhibitor TNP-470 on accelerated hepatocellular carcinoma (HCC) growth in the regenerating liver. Methods. After 70% partial hepatectomy (PH), AH-130 cells were injected into the portal vein of Donryu rats. A control group was given the vehicle only, and the treated group was given 10mg/kg TNP-470 subcutaneously every second day, from 24 h after tumor implantation, seven times. On day 14, tumor growth was evaluated by the number of foci on the liver surface, liver weight, and the microvessel density of the tumor. Results. The number of foci was significantly less in the treated group (116.5 ± 103.1) than in the control group (319.3 ± 223.1) (P <0.05), as was microvessel density, which was 31.3 ± 14.0/mm2 in the treated group and 61.2 ± 18.9/mm2 in the control group (P <0.05). The liver tended to weigh less in the treated group (12.15 ± 1.28 g) than in the control group (15.22 ± 5.35 g). We also assessed whether TNP-470 retards liver regeneration. Seven days after 70% PH, the liver weight in the treated group was similar to that in the control group. Total bilirubin, serum glutamic oxaloacetic transaminase, and serum glutamic pyruvic transaminase were not higher in the treated group than in the control group. Conclusion. TNP-470 can suppress HCC growth without retarding liver regeneration after PH.

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