Analysis on the cleavage of membrane-bound betacellulin

Hiroko Tada, Masaharu Seno, Y. Kawaguchi, R. Sasada, K. Igarashi, H. Yamada

Research output: Contribution to journalArticle

Abstract

Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, is synthesized as a membrane-bound form which is cleaved to a released form. This represents the switching juxtacrine function of BTC to paracrine. To analyze the mechanism of cleavage, we established a series of recombinant cells that produced an alkaline phosphatase (ALP) fused to a part of membrane-bound BTC using Chinese hamster Ovary (CHO) cells and mouse fibroblast cell lines (A9 and Balb/c 3T3 A31). In CHO transfectants, about 90% of total ALP activity was retained on the cells, whereas A9 and Balb/c 3T3 A31 transfectants extensively secreted the ALP activity (about 40%). None of the stimulants, including a phorbor ester (PMA), A23187, retinoic acid, cAMP, cGMP, fibroblasl growth factor, EGF and BTC its self, enhanced the cleavage and release of ALP-BTC in these transfectants. These results suggest that the cleavage of BTC is regulated in different ways from those of other members of EGF-family, such as transforming growth factor a and heparin-binding EGF-like growth factor, whose cleavage are excellently stimulated with PMA.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number3
Publication statusPublished - 1996

Fingerprint

epidermal growth factor
alkaline phosphatase
Membranes
Epidermal Growth Factor
Alkaline Phosphatase
Chinese hamsters
growth factors
Cricetulus
transforming growth factors
Ovary
retinoic acid
Intercellular Signaling Peptides and Proteins
heparin
cells
Cells
fibroblasts
Switching functions
esters
cell lines
Calcimycin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Tada, H., Seno, M., Kawaguchi, Y., Sasada, R., Igarashi, K., & Yamada, H. (1996). Analysis on the cleavage of membrane-bound betacellulin. FASEB Journal, 10(3).

Analysis on the cleavage of membrane-bound betacellulin. / Tada, Hiroko; Seno, Masaharu; Kawaguchi, Y.; Sasada, R.; Igarashi, K.; Yamada, H.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Tada, H, Seno, M, Kawaguchi, Y, Sasada, R, Igarashi, K & Yamada, H 1996, 'Analysis on the cleavage of membrane-bound betacellulin', FASEB Journal, vol. 10, no. 3.
Tada H, Seno M, Kawaguchi Y, Sasada R, Igarashi K, Yamada H. Analysis on the cleavage of membrane-bound betacellulin. FASEB Journal. 1996;10(3).
Tada, Hiroko ; Seno, Masaharu ; Kawaguchi, Y. ; Sasada, R. ; Igarashi, K. ; Yamada, H. / Analysis on the cleavage of membrane-bound betacellulin. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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AU - Seno, Masaharu

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AU - Sasada, R.

AU - Igarashi, K.

AU - Yamada, H.

PY - 1996

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