Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction

Kewei Bi; Lei Shi; Yoko Maehara; Shin Ichi Miyoshi; Ken Ichi Tomochika; Sumio Shinoda

doi:10.1111/j.1348-0421.2000.tb01258.x

Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction

Kewei Bi, Lei Shi, Yoko Maehara, Shin Ichi Miyoshi, Ken Ichi Tomochika, Sumio Shinoda

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

A total of 51 Vibrio mimicus clinical strains from different geographic locations were examined by arbitrarily primed polymerase chain reaction (AP- PCR). The primer VMH-3 divided them into 28 groups, although 18 groups consisted of a single strain at present. All groups had a common 1.0-kb amplification fragment. Most of the groups consisted of strains from same region, although two exceptional groups showed a few amplification fragments including strains from different regions. AP-PCR groups were not consistently associated with serogroups. AP-PCR is thought to be a valuable and easy method for the epidemiological study of V. mimicus.

Original language	English
Pages (from-to)	149-153
Number of pages	5
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	44
Issue number	2
DOIs	https://doi.org/10.1111/j.1348-0421.2000.tb01258.x
Publication status	Published - Jan 1 2000

Keywords

Arbitrarily primed polymerase chain reaction
Vibrio mimicus

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.2000.tb01258.x

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Bi, K., Shi, L., Maehara, Y., Miyoshi, S. I., Tomochika, K. I., & Shinoda, S. (2000). Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction. MICROBIOLOGY and IMMUNOLOGY, 44(2), 149-153. https://doi.org/10.1111/j.1348-0421.2000.tb01258.x

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