Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction

Kewei Bi, Lei Shi, Yoko Maehara, Shin Ichi Miyoshi, Ken Ichi Tomochika, Sumio Shinoda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A total of 51 Vibrio mimicus clinical strains from different geographic locations were examined by arbitrarily primed polymerase chain reaction (AP- PCR). The primer VMH-3 divided them into 28 groups, although 18 groups consisted of a single strain at present. All groups had a common 1.0-kb amplification fragment. Most of the groups consisted of strains from same region, although two exceptional groups showed a few amplification fragments including strains from different regions. AP-PCR groups were not consistently associated with serogroups. AP-PCR is thought to be a valuable and easy method for the epidemiological study of V. mimicus.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalMICROBIOLOGY and IMMUNOLOGY
Volume44
Issue number2
DOIs
Publication statusPublished - 2000

Keywords

  • Arbitrarily primed polymerase chain reaction
  • Vibrio mimicus

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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