Abstract
We have previously reported the graft transmission of target specificity for RNA silencing using transgenic Nicotiana benthamiana plants expressing the coat protein gene (CP, including the 3′ non-translated region) of Sweet potato feathery mottle virus. Transgenic plants carrying the 5′ 200 and 400 bp regions of CP were newly produced. From these plants, two silenced and two non-silenced lines were selected to investigate the manifestation of transitive RNA silencing by graft experiments. Non-silenced scions carrying the entire transgene were grafted onto either 5′ or 3′ silencing inducer rootstocks. When non-silenced scions were grafted onto 5′ silencing inducer rootstocks, RNA silencing was induced in the non-silenced scions and spread toward the 3′ region of the transgene mRNA. Similarly, when non-silenced scions were grafted onto 3′ silencing inducer rootstocks, RNA silencing was induced in the non-silenced scions, but was restricted to the 3′ region of the transgene and did not spread to the 5′ region. In addition, results from crossing experiments, involving non-silenced and 3′ silencing inducer plants, confirmed the above finding. This indicates that RNA silencing spreads in the 5′-3′ direction, not in the 3′-5′ direction, along the transgene mRNA.
| Original language | English |
|---|---|
| Pages (from-to) | 35-47 |
| Number of pages | 13 |
| Journal | Plant Molecular Biology |
| Volume | 63 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2007 |
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Keywords
- Coat protein gene (CP)
- Grafting
- Nicotiana benthamiana
- Post-transcriptional gene silencing
- RNA silencing
- siRNA
- Sweet potato feathery mottle virus
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
Cite this
Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of Sweet potato feathery mottle virus. / Haque, A. K M N; Tanaka, Yoshikazu; Sonoda, Shoji; Nishiguchi, Masamichi.
In: Plant Molecular Biology, Vol. 63, No. 1, 01.2007, p. 35-47.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of Sweet potato feathery mottle virus
AU - Haque, A. K M N
AU - Tanaka, Yoshikazu
AU - Sonoda, Shoji
AU - Nishiguchi, Masamichi
PY - 2007/1
Y1 - 2007/1
N2 - We have previously reported the graft transmission of target specificity for RNA silencing using transgenic Nicotiana benthamiana plants expressing the coat protein gene (CP, including the 3′ non-translated region) of Sweet potato feathery mottle virus. Transgenic plants carrying the 5′ 200 and 400 bp regions of CP were newly produced. From these plants, two silenced and two non-silenced lines were selected to investigate the manifestation of transitive RNA silencing by graft experiments. Non-silenced scions carrying the entire transgene were grafted onto either 5′ or 3′ silencing inducer rootstocks. When non-silenced scions were grafted onto 5′ silencing inducer rootstocks, RNA silencing was induced in the non-silenced scions and spread toward the 3′ region of the transgene mRNA. Similarly, when non-silenced scions were grafted onto 3′ silencing inducer rootstocks, RNA silencing was induced in the non-silenced scions, but was restricted to the 3′ region of the transgene and did not spread to the 5′ region. In addition, results from crossing experiments, involving non-silenced and 3′ silencing inducer plants, confirmed the above finding. This indicates that RNA silencing spreads in the 5′-3′ direction, not in the 3′-5′ direction, along the transgene mRNA.
AB - We have previously reported the graft transmission of target specificity for RNA silencing using transgenic Nicotiana benthamiana plants expressing the coat protein gene (CP, including the 3′ non-translated region) of Sweet potato feathery mottle virus. Transgenic plants carrying the 5′ 200 and 400 bp regions of CP were newly produced. From these plants, two silenced and two non-silenced lines were selected to investigate the manifestation of transitive RNA silencing by graft experiments. Non-silenced scions carrying the entire transgene were grafted onto either 5′ or 3′ silencing inducer rootstocks. When non-silenced scions were grafted onto 5′ silencing inducer rootstocks, RNA silencing was induced in the non-silenced scions and spread toward the 3′ region of the transgene mRNA. Similarly, when non-silenced scions were grafted onto 3′ silencing inducer rootstocks, RNA silencing was induced in the non-silenced scions, but was restricted to the 3′ region of the transgene and did not spread to the 5′ region. In addition, results from crossing experiments, involving non-silenced and 3′ silencing inducer plants, confirmed the above finding. This indicates that RNA silencing spreads in the 5′-3′ direction, not in the 3′-5′ direction, along the transgene mRNA.
KW - Coat protein gene (CP)
KW - Grafting
KW - Nicotiana benthamiana
KW - Post-transcriptional gene silencing
KW - RNA silencing
KW - siRNA
KW - Sweet potato feathery mottle virus
UR - http://www.scopus.com/inward/record.url?scp=33845643721&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845643721&partnerID=8YFLogxK
U2 - 10.1007/s11103-006-9070-6
DO - 10.1007/s11103-006-9070-6
M3 - Article
VL - 63
SP - 35
EP - 47
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 1
ER -