Analysis of transcytosis of CCN2 by chondrocytes

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Transcytosis is a mechanism for the transcellular transport of biomolecules. Analysis of transcytosis is frequently performed in cells with distinct polarity, such as brain endothelial cells. However, in cells without evident polarity, analysis of transcytosis has not been performed. Here, we describe a method for analyzing transcytosis of a CCN family protein through chondrocytic cells having no apparent polarity.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages405-413
Number of pages9
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)10643745

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Keywords

  • Analysis of transcytosis
  • Cells without polarity
  • Chondrocytes
  • FLAG-tagged recombinant CCN2 protein
  • Transwell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kawata, K., Kubota, S., & Takigawa, M. (2017). Analysis of transcytosis of CCN2 by chondrocytes. In Methods in Molecular Biology (Vol. 1489, pp. 405-413). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_33