TY - JOUR
T1 - Analysis of tissue-specific expression of arabidopsis thaliana HSP90-family gene HSP81
AU - Yabe, Naoto
AU - Takahashi, Taku
AU - Komeda, Yoshibumi
N1 - Funding Information:
We would like to thank Dr. J.T. Mulligan and Dr. R.W. Davis of Stanford University for Arabidopsis AGEM11 genomic library. This work was supported in part by fellowships of the Japan Society for the Promotion of Science for Japanese Junior Scientists.
PY - 1994
Y1 - 1994
N2 - We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures.Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22°C. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a rβ-glucuronidase (GUS) gene, confirmed these results. At 22°C, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35°C treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants.Exogenous application of various chemicals such as ABA, GA3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22° C; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.
AB - We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures.Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22°C. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a rβ-glucuronidase (GUS) gene, confirmed these results. At 22°C, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35°C treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants.Exogenous application of various chemicals such as ABA, GA3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22° C; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.
KW - Arabidopsis
KW - Developmental regulation
KW - HSP81
KW - HSP90 gene family
KW - Tissue specific expression
KW - Transgenic plants
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U2 - 10.1093/oxfordjournals.pcp.a078715
DO - 10.1093/oxfordjournals.pcp.a078715
M3 - Article
C2 - 7697294
AN - SCOPUS:0028675649
VL - 35
SP - 1207
EP - 1219
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 8
ER -