Analysis of tissue-specific expression of arabidopsis thaliana HSP90-family gene HSP81

Naoto Yabe, Taku Takahashi, Yoshibumi Komeda

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures.Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22°C. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a rβ-glucuronidase (GUS) gene, confirmed these results. At 22°C, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35°C treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants.Exogenous application of various chemicals such as ABA, GA3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22° C; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.

Original languageEnglish
Pages (from-to)1207-1219
Number of pages13
JournalPlant and Cell Physiology
Volume35
Issue number8
DOIs
Publication statusPublished - Jan 1 1994
Externally publishedYes

Keywords

  • Arabidopsis
  • Developmental regulation
  • HSP81
  • HSP90 gene family
  • Tissue specific expression
  • Transgenic plants

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Analysis of tissue-specific expression of arabidopsis thaliana HSP90-family gene HSP81'. Together they form a unique fingerprint.

  • Cite this