Abstract
We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures.Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22°C. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a rβ-glucuronidase (GUS) gene, confirmed these results. At 22°C, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35°C treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants.Exogenous application of various chemicals such as ABA, GA3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22° C; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 1207-1219 |
| Number of pages | 13 |
| Journal | Plant and Cell Physiology |
| Volume | 35 |
| Issue number | 8 |
| Publication status | Published - 1994 |
| Externally published | Yes |
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Keywords
- Arabidopsis
- Developmental regulation
- HSP81
- HSP90 gene family
- Tissue specific expression
- Transgenic plants
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Ecology
- Cell Biology
- Physiology
- Plant Science
Cite this
Analysis of tissue-specific expression of arabidopsis thaliana HSP90-family gene HSP81. / Yabe, Naoto; Takahashi, Taku; Komeda, Yoshibumi.
In: Plant and Cell Physiology, Vol. 35, No. 8, 1994, p. 1207-1219.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of tissue-specific expression of arabidopsis thaliana HSP90-family gene HSP81
AU - Yabe, Naoto
AU - Takahashi, Taku
AU - Komeda, Yoshibumi
PY - 1994
Y1 - 1994
N2 - We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures.Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22°C. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a rβ-glucuronidase (GUS) gene, confirmed these results. At 22°C, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35°C treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants.Exogenous application of various chemicals such as ABA, GA3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22° C; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.
AB - We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures.Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22°C. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a rβ-glucuronidase (GUS) gene, confirmed these results. At 22°C, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35°C treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants.Exogenous application of various chemicals such as ABA, GA3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22° C; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.
KW - Arabidopsis
KW - Developmental regulation
KW - HSP81
KW - HSP90 gene family
KW - Tissue specific expression
KW - Transgenic plants
UR - http://www.scopus.com/inward/record.url?scp=0028675649&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028675649&partnerID=8YFLogxK
M3 - Article
VL - 35
SP - 1207
EP - 1219
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 8
ER -