Analysis of tissue-specific expression of arabidopsis thaliana HSP90-family gene HSP81

Naoto Yabe, Taku Takahashi, Yoshibumi Komeda

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures.Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22°C. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a rβ-glucuronidase (GUS) gene, confirmed these results. At 22°C, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35°C treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants.Exogenous application of various chemicals such as ABA, GA3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22° C; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.

Original languageEnglish
Pages (from-to)1207-1219
Number of pages13
JournalPlant and Cell Physiology
Volume35
Issue number8
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

transgenic plant
Arabidopsis Thaliana
tissue analysis
Glucuronidase
Arabidopsis
transgenic plants
Arabidopsis thaliana
Genes
Roots
Messenger RNA
Genetically Modified Plants
Tissue
Gene
gene
Pathway
genes
Heat
Kinetin
In Situ Hybridization
Pollen

Keywords

  • Arabidopsis
  • Developmental regulation
  • HSP81
  • HSP90 gene family
  • Tissue specific expression
  • Transgenic plants

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Analysis of tissue-specific expression of arabidopsis thaliana HSP90-family gene HSP81. / Yabe, Naoto; Takahashi, Taku; Komeda, Yoshibumi.

In: Plant and Cell Physiology, Vol. 35, No. 8, 1994, p. 1207-1219.

Research output: Contribution to journalArticle

@article{cf8b33f187a141119903b2d801c9f972,
title = "Analysis of tissue-specific expression of arabidopsis thaliana HSP90-family gene HSP81",
abstract = "We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures.Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22°C. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a rβ-glucuronidase (GUS) gene, confirmed these results. At 22°C, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35°C treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants.Exogenous application of various chemicals such as ABA, GA3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22° C; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.",
keywords = "Arabidopsis, Developmental regulation, HSP81, HSP90 gene family, Tissue specific expression, Transgenic plants",
author = "Naoto Yabe and Taku Takahashi and Yoshibumi Komeda",
year = "1994",
language = "English",
volume = "35",
pages = "1207--1219",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "8",

}

TY - JOUR

T1 - Analysis of tissue-specific expression of arabidopsis thaliana HSP90-family gene HSP81

AU - Yabe, Naoto

AU - Takahashi, Taku

AU - Komeda, Yoshibumi

PY - 1994

Y1 - 1994

N2 - We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures.Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22°C. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a rβ-glucuronidase (GUS) gene, confirmed these results. At 22°C, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35°C treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants.Exogenous application of various chemicals such as ABA, GA3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22° C; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.

AB - We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures.Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22°C. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a rβ-glucuronidase (GUS) gene, confirmed these results. At 22°C, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35°C treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants.Exogenous application of various chemicals such as ABA, GA3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22° C; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.

KW - Arabidopsis

KW - Developmental regulation

KW - HSP81

KW - HSP90 gene family

KW - Tissue specific expression

KW - Transgenic plants

UR - http://www.scopus.com/inward/record.url?scp=0028675649&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028675649&partnerID=8YFLogxK

M3 - Article

C2 - 7697294

AN - SCOPUS:0028675649

VL - 35

SP - 1207

EP - 1219

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 8

ER -