Analysis of the structural gene encoding a hemolysin in Vibrio mimicus

Md Monzur Rahman, Shin-ichi Miyoshi, Ken Ichi Tomochika, Hitoshi Wakae, Sumio Shinoda

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

An environmental isolate of V. mimicus, strain E-33, has been reported to produce and secrete a hemolysin of 63 kDa. The hemolysin is enterotoxic in test animals. The nucleotide sequence of the structural gene of the hemolysin was determined. We found a 2,232 bp open reading frame, which codes a peptide of 744 amino acids, with a calculated molecular weight of 83,903 Da. The sequence for the structural gene was closely related to the V. cholerae el tor hlyA gene, coding an exocellular hemolysin. The amino terminal amino- acid sequence of the 63 kDa hemolysin, purified from V. mimicus, was determined by the Edman degradation method and found to be NH2-S-V-S-A-N-N- V-T-N-N-N-E-T. This sequence is identical from S-152 to T-164 predicted from the nucleotide sequence. So, it seems that the mature hemolysin in V. mimicus is processed upon deleting the first 151 amino acids, and the molecular mass is 65,972 Da. Analyzing the deduced amino-acid sequence, we also found a potential signal sequence of 24 amino acids at the amino terminal. Our results suggest that, like V. cholerae hemolysin, two-step processing also exists in V. mimicus hemolysin.

Original languageEnglish
Pages (from-to)169-173
Number of pages5
JournalMicrobiology and Immunology
Volume41
Issue number2
Publication statusPublished - 1997

Fingerprint

Hemolysin Proteins
Genes
Cholera
Amino Acids
Vibrio mimicus
Amino Acid Sequence
Protein Sorting Signals
Open Reading Frames
Molecular Weight
Vibrio hemolysin
Peptides

Keywords

  • Hemolysin
  • Structural gene
  • Vibrio mimicus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Rahman, M. M., Miyoshi, S., Tomochika, K. I., Wakae, H., & Shinoda, S. (1997). Analysis of the structural gene encoding a hemolysin in Vibrio mimicus. Microbiology and Immunology, 41(2), 169-173.

Analysis of the structural gene encoding a hemolysin in Vibrio mimicus. / Rahman, Md Monzur; Miyoshi, Shin-ichi; Tomochika, Ken Ichi; Wakae, Hitoshi; Shinoda, Sumio.

In: Microbiology and Immunology, Vol. 41, No. 2, 1997, p. 169-173.

Research output: Contribution to journalArticle

Rahman, MM, Miyoshi, S, Tomochika, KI, Wakae, H & Shinoda, S 1997, 'Analysis of the structural gene encoding a hemolysin in Vibrio mimicus', Microbiology and Immunology, vol. 41, no. 2, pp. 169-173.
Rahman, Md Monzur ; Miyoshi, Shin-ichi ; Tomochika, Ken Ichi ; Wakae, Hitoshi ; Shinoda, Sumio. / Analysis of the structural gene encoding a hemolysin in Vibrio mimicus. In: Microbiology and Immunology. 1997 ; Vol. 41, No. 2. pp. 169-173.
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