Analysis of the Genome of an Epstein-Barr-Virus (EBV)-Related Herpesvirus in a Cynomolgus Monkey Cell Line (Si-IIA)

Hideo Ino, Kazuhiko Hayashi, Hiroyuki Yanai, Norihiro Teramoto, Tirtha Raj Koirala, Hong Li Chen, Takashi Oka, Tadashi Yoshino, Kiyoshi Takahashi, Tadaatsu Akagi

Research output: Contribution to journalArticle

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Abstract

A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalActa Medica Okayama
Volume51
Issue number4
Publication statusPublished - Aug 1997

Fingerprint

Macaca fascicularis
Herpesviridae
Human Herpesvirus 4
Viruses
Genes
Cells
Genome
Cell Line
DNA
Polymerase Chain Reaction
DNA Primers
Polymerase chain reaction
B-Cell Lymphoma
Southern Blotting
Haplorhini
Lymphoma
Nucleotides
Rabbits

Keywords

  • Epstein-Barr virus
  • HVMF I
  • Lymphoma
  • Monkey cell line
  • PCR

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Analysis of the Genome of an Epstein-Barr-Virus (EBV)-Related Herpesvirus in a Cynomolgus Monkey Cell Line (Si-IIA). / Ino, Hideo; Hayashi, Kazuhiko; Yanai, Hiroyuki; Teramoto, Norihiro; Koirala, Tirtha Raj; Chen, Hong Li; Oka, Takashi; Yoshino, Tadashi; Takahashi, Kiyoshi; Akagi, Tadaatsu.

In: Acta Medica Okayama, Vol. 51, No. 4, 08.1997, p. 207-212.

Research output: Contribution to journalArticle

Ino, H, Hayashi, K, Yanai, H, Teramoto, N, Koirala, TR, Chen, HL, Oka, T, Yoshino, T, Takahashi, K & Akagi, T 1997, 'Analysis of the Genome of an Epstein-Barr-Virus (EBV)-Related Herpesvirus in a Cynomolgus Monkey Cell Line (Si-IIA)', Acta Medica Okayama, vol. 51, no. 4, pp. 207-212.
Ino, Hideo ; Hayashi, Kazuhiko ; Yanai, Hiroyuki ; Teramoto, Norihiro ; Koirala, Tirtha Raj ; Chen, Hong Li ; Oka, Takashi ; Yoshino, Tadashi ; Takahashi, Kiyoshi ; Akagi, Tadaatsu. / Analysis of the Genome of an Epstein-Barr-Virus (EBV)-Related Herpesvirus in a Cynomolgus Monkey Cell Line (Si-IIA). In: Acta Medica Okayama. 1997 ; Vol. 51, No. 4. pp. 207-212.
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