Analysis of the genetic information of a DNA segment of a new virus from silkworm

H. Bando, Tohru Hayakawa, S. Asano, K. Sahara, M. Nakagaki, T. Iizuka

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

In 1983, a parvo-like virus (Yamanashi isolate) was newly isolated from silkworm. However, unlike parvovirus, two DNA molecules (VD1 and 2) were always extracted from purified virions. To investigate the structure and organization of the virus genomes, we determined the complete nucleotide sequence of VD2. The sequence consisted of 6031 nucleotides (nts) and contained a large open reading frame (ORF1) with 3513 nts. A smaller open reading frame (ORF2) with 702 nts was found in the complementary sequence. Computer analysis revealed that both ORFs did not code for the major structural proteins (VP1, 2, 3, and 4). These results suggest that VD2 has not enough information to produce progeny virions by itself. Further, the structural importance of the terminal sequence (CTS) common to both VD1 and VD2 was also predicted by a computer analysis.

Original languageEnglish
Pages (from-to)1147-1155
Number of pages9
JournalArchives of Virology
Volume140
Issue number6
DOIs
Publication statusPublished - Jun 1995
Externally publishedYes

Fingerprint

Bombyx
Open Reading Frames
Nucleotides
Viruses
Virion
DNA
Viral Structures
Parvovirus
Genome
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Analysis of the genetic information of a DNA segment of a new virus from silkworm. / Bando, H.; Hayakawa, Tohru; Asano, S.; Sahara, K.; Nakagaki, M.; Iizuka, T.

In: Archives of Virology, Vol. 140, No. 6, 06.1995, p. 1147-1155.

Research output: Contribution to journalArticle

Bando, H, Hayakawa, T, Asano, S, Sahara, K, Nakagaki, M & Iizuka, T 1995, 'Analysis of the genetic information of a DNA segment of a new virus from silkworm', Archives of Virology, vol. 140, no. 6, pp. 1147-1155. https://doi.org/10.1007/BF01315423
Bando, H. ; Hayakawa, Tohru ; Asano, S. ; Sahara, K. ; Nakagaki, M. ; Iizuka, T. / Analysis of the genetic information of a DNA segment of a new virus from silkworm. In: Archives of Virology. 1995 ; Vol. 140, No. 6. pp. 1147-1155.
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