Analysis of the cytokine interaction among interleukin-1β, interleukin- 8, and interleukin-1 receptor antagonist in the rabbit ovulatory process

Takeshi Ujioka, Akihiro Matsukawa, Nobuyuki Tanaka, Kohei Matsuura, Masaru Yoshinaga, Hitoshi Okamura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: To elucidate the regulation and involvement of interleukin (IL)-1β, IL-8, and IL-I receptor antagonist in the hCG-induced rabbit ovulatory process. Design: Randomized, controlled animal study. Setting: University research laboratory. Animal(s): Mature female New Zealand white rabbits. Intervention(s): After IV administration of 100 IU of hCG to rabbits, ovarian levels of IL-1β, IL-8, and IL-I receptor antagonist were determined at indicated times by ELISA. Anti-IL- 1β, anti-IL-8, or anti-IL- 1 receptor antagonist antiserum was given IV 30 minutes before hCG injection. Main Outcome Measure(s): Effects of each antiserum on the levels of the other cytokines and neutrophil accumulation, assessed by myeloperoxidase activity, were determined. Ovulation rate (rate of ruptured follicles) was also evaluated. Result(s): The maximal level of IL-8 was detected at 4 hours, which preceded that of IL-1β and IL-1 receptor antagonist, detected at 6 hours after hCG injection. Administration of anti-IL-1β antiserum resulted in a statistically significant reduction of the peak levels of IL-8 and IL-I receptor antagonist. Administration of anti-IL-8 antiserum reduced the accumulation of IL-1β and IL-1 receptor antagonist. Anti-IL-1 receptor antagonist antiserum significantly augmented the accumulation of IL-1β and IL-8. Myeloperoxidase activity was reduced by anti-IL-8 antiserum. Anti-IL- 1β and anti-IL-8 antiserum reduced the hCG-induced ovulation rate, but a synergistic effect was nol evident when these antisera were injected simultaneously. Anti-IL-1 receptor antagonist antiserum had no apparent effect on ovulatory efficiency. Conclusion(s): IL-1β, IL-8, and IL-1 receptor antagonist may affect the accumulation of related cytokines in ovaries and may be involved in ovulation.

Original languageEnglish
Pages (from-to)759-765
Number of pages7
JournalFertility and Sterility
Volume70
Issue number4
DOIs
Publication statusPublished - Oct 1998
Externally publishedYes

Fingerprint

Interleukin-1 Receptors
Interleukin-8
Interleukin-1
Cytokines
Rabbits
Immune Sera
Interleukin Receptors
Ovulation
Peroxidase
Injections
Ovary
Neutrophils
Enzyme-Linked Immunosorbent Assay
Outcome Assessment (Health Care)

Keywords

  • IL-1β
  • IL-1Ra
  • IL-8
  • Ovulation
  • Rabbit

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Analysis of the cytokine interaction among interleukin-1β, interleukin- 8, and interleukin-1 receptor antagonist in the rabbit ovulatory process. / Ujioka, Takeshi; Matsukawa, Akihiro; Tanaka, Nobuyuki; Matsuura, Kohei; Yoshinaga, Masaru; Okamura, Hitoshi.

In: Fertility and Sterility, Vol. 70, No. 4, 10.1998, p. 759-765.

Research output: Contribution to journalArticle

Ujioka, Takeshi ; Matsukawa, Akihiro ; Tanaka, Nobuyuki ; Matsuura, Kohei ; Yoshinaga, Masaru ; Okamura, Hitoshi. / Analysis of the cytokine interaction among interleukin-1β, interleukin- 8, and interleukin-1 receptor antagonist in the rabbit ovulatory process. In: Fertility and Sterility. 1998 ; Vol. 70, No. 4. pp. 759-765.
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AU - Matsuura, Kohei

AU - Yoshinaga, Masaru

AU - Okamura, Hitoshi

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AB - Objective: To elucidate the regulation and involvement of interleukin (IL)-1β, IL-8, and IL-I receptor antagonist in the hCG-induced rabbit ovulatory process. Design: Randomized, controlled animal study. Setting: University research laboratory. Animal(s): Mature female New Zealand white rabbits. Intervention(s): After IV administration of 100 IU of hCG to rabbits, ovarian levels of IL-1β, IL-8, and IL-I receptor antagonist were determined at indicated times by ELISA. Anti-IL- 1β, anti-IL-8, or anti-IL- 1 receptor antagonist antiserum was given IV 30 minutes before hCG injection. Main Outcome Measure(s): Effects of each antiserum on the levels of the other cytokines and neutrophil accumulation, assessed by myeloperoxidase activity, were determined. Ovulation rate (rate of ruptured follicles) was also evaluated. Result(s): The maximal level of IL-8 was detected at 4 hours, which preceded that of IL-1β and IL-1 receptor antagonist, detected at 6 hours after hCG injection. Administration of anti-IL-1β antiserum resulted in a statistically significant reduction of the peak levels of IL-8 and IL-I receptor antagonist. Administration of anti-IL-8 antiserum reduced the accumulation of IL-1β and IL-1 receptor antagonist. Anti-IL-1 receptor antagonist antiserum significantly augmented the accumulation of IL-1β and IL-8. Myeloperoxidase activity was reduced by anti-IL-8 antiserum. Anti-IL- 1β and anti-IL-8 antiserum reduced the hCG-induced ovulation rate, but a synergistic effect was nol evident when these antisera were injected simultaneously. Anti-IL-1 receptor antagonist antiserum had no apparent effect on ovulatory efficiency. Conclusion(s): IL-1β, IL-8, and IL-1 receptor antagonist may affect the accumulation of related cytokines in ovaries and may be involved in ovulation.

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