Analysis of the 22-NBD-cholesterol transfer between liposome membranes and its relation to the intermembrane exchange of 25-hydroxycholesterol

Haruyuki Ishii, Toshinori Shimanouchi, Hiroshi Umakoshi, Peter Walde, Ryoichi Kuboi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The transfer of 22-NBD-cholesterol (22-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-23,24-bisnor-5-cholen-3-ol) between two liposome membranes was quantitatively analyzed by using the fluorescence resonance energy transfer (FRET) method. Liposomes labeled with both 22-NBD-cholesterol and a rhodamine-labeled phosphatidylethanolamine (Rh-DHPE) were used as donor liposomes, and the 22-NBD-cholesterol transfer from these donor liposomes to acceptor liposomes prepared from same type of phosphatidylcholine was monitored. The transfer kinetics was found to be composed of a fast and a slow phase, and all kinetic measurements could be fitted with a bi-exponential model. The results obtained indicate that the 22-NBD-cholesterol transfer kinetics between liposome membranes depends on the fluidity of the liposome used and that the curvature may affect the kinetics. Furthermore, the behavior of 22-NBD-cholesterol in lipid membrane is similar to that of the oxysterol 25-hydroxycholesterol rather than cholesterol. It is proposed that 22-NBD-cholesterol can be a useful fluorescent probe to mimic the intermembrane transfer of oxidized cholesterols like 25-hydroxycholesterol, rather than that of cholesterol itself.

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume77
Issue number1
DOIs
Publication statusPublished - May 1 2010
Externally publishedYes

Fingerprint

Liposomes
Cholesterol
cholesterol
membranes
Membranes
Kinetics
kinetics
Fluorescence Resonance Energy Transfer
Rhodamines
Fluidity
Membrane Lipids
7-nitrobenz-2-oxa-1,3-diazol-4-ylcholesterol
25-hydroxycholesterol
Phosphatidylcholines
Fluorescent Dyes
resonance fluorescence
rhodamine
lipids
energy transfer
curvature

Keywords

  • 22-NBD-cholesterol
  • Fluorescence resonance energy transfer
  • Intermembrane transfer
  • Liposome membranes
  • Oxidized cholesterol

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Analysis of the 22-NBD-cholesterol transfer between liposome membranes and its relation to the intermembrane exchange of 25-hydroxycholesterol. / Ishii, Haruyuki; Shimanouchi, Toshinori; Umakoshi, Hiroshi; Walde, Peter; Kuboi, Ryoichi.

In: Colloids and Surfaces B: Biointerfaces, Vol. 77, No. 1, 01.05.2010, p. 117-121.

Research output: Contribution to journalArticle

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