Analysis of T-DNA-mediated translational β-glucuronidase gene fusions

Sunee Kertbundit, Rosario Linacero, Pierre Rouzé, Ivan Galis, Jiri Macas, Francine Deboeck, Suzy Renckens, Jean Pierre Hernalsteens, Henri De Greve

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6 Citations (Scopus)

Abstract

Three random translational β-glucuronidase (gus) gene fusions were previously obtained in Arabidopsis thaliana, using Agrobacterium-mediated transfer of a gus coding sequence without promoter and ATG initiation site. These were analysed by IPCR amplification of the sequence of gus and nucleotide sequence analysis. In one instance, the gus sequence was fused, in inverse orientation, to the nos promoter sequence of a truncated tandem T-DNA copy and translated from a spurious ATG in this sequence. In the second transgenic line, the gus gene was fused to A. thaliana DNA, 27 bp downstream an ATG. In this line, a large deletion occurred at the target site of the T-DNA. In the third line, gus is fused in frame to a plant DNA sequence after the eighth codon of an open reading frame encoding a protein of 619 amino acids. This protein has significant homology with animal and plant (receptor) serine/threonine protein kinases. The twelve subdomains essential for kinase activity are conserved. The presence of a potential signal peptide and a membrane-spanning domain suggest that it may be a receptor kinase. These data confirm that plant genes can be tagged as functional translational gene fusions.

Original languageEnglish
Pages (from-to)205-217
Number of pages13
JournalPlant Molecular Biology
Volume36
Issue number2
DOIs
Publication statusPublished - Jan 1 1998

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Keywords

  • Agrobacterium tumefaciens
  • Arabidopsis thaliana
  • Gene fusion
  • Hybrid proteins
  • Insertion mutagenesis
  • Serine/threonine receptor kinase

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Kertbundit, S., Linacero, R., Rouzé, P., Galis, I., Macas, J., Deboeck, F., Renckens, S., Hernalsteens, J. P., & De Greve, H. (1998). Analysis of T-DNA-mediated translational β-glucuronidase gene fusions. Plant Molecular Biology, 36(2), 205-217. https://doi.org/10.1023/A:1005902730810