Analysis of stimulants and related compounds by automated on-line in-tube SPME/LC/MS

Hiroyuki Kataoka, Shizuo Narimatsu, Heather L. Lord, Janusz Pawliszyn

Research output: Contribution to journalArticle

Abstract

A simple and rapid method for the analysis of amphetamines and their 3,4-methylenedioxy derivatives was developed by automated in-tube solid-phase microextraction/liquid chromatography/electrospray ionization mass spectrometry (in-tube SPME/LC/ESI-MS). Optimum In-tube SPME conditions for these drugs were 15 draw/eject cycles of 35 μL of sample in Tris-HCl (pH 8.5) using Omegawax 250 capillary column. The drugs extracted by the capillary column were easily desorbed by mobile phase flow, and carryover was not observed. Using in-tube SPME/LC/ESI-MS with selected ion monitoring, the calibration curves of these drugs were linear in the range of 2-100 ng/mL with correlation coefficients above 0.9985 (n=18), and the detection limits (S/N=3) were 0.4-0.8 ng/mL. This method was successfully applied to the analysis of urine samples without interference peaks and with recoveries above 80%.

Original languageEnglish
Pages (from-to)150-151
Number of pages2
JournalJapanese Journal of Forensic Toxicology
Volume18
Issue number2
Publication statusPublished - Dec 1 2000

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology

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    Kataoka, H., Narimatsu, S., Lord, H. L., & Pawliszyn, J. (2000). Analysis of stimulants and related compounds by automated on-line in-tube SPME/LC/MS. Japanese Journal of Forensic Toxicology, 18(2), 150-151.