Analysis of signaling pathways activated by CCN proteins

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

CCN family proteins activate multiple intracellular phosphorylated kinase cascades to yield the multiple physiological functions of a variety of target cells. In this chapter, we describe our protocol examining the effects of these proteins on signal transduction pathways, especially mitogen-activated protein kinase cascades, activated by CCN member proteins, which examinations have been carried out mainly by using Western blotting methodologies.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages139-143
Number of pages5
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)10643745

Fingerprint

CCN Intercellular Signaling Proteins
Mitogen-Activated Protein Kinases
Signal Transduction
Proteins
Phosphotransferases
Western Blotting

Keywords

  • CCN signaling
  • ERK1/2 (p44/42 MAPK)
  • JNK
  • MAPK pathway
  • p38 MAPK
  • PI3K-PKB/Akt pathway
  • Western blotting

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kawaki, H., Kubota, S., & Takigawa, M. (2017). Analysis of signaling pathways activated by CCN proteins. In Methods in Molecular Biology (Vol. 1489, pp. 139-143). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_14

Analysis of signaling pathways activated by CCN proteins. / Kawaki, Harumi; Kubota, Satoshi; Takigawa, Masaharu.

Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. p. 139-143 (Methods in Molecular Biology; Vol. 1489).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kawaki, H, Kubota, S & Takigawa, M 2017, Analysis of signaling pathways activated by CCN proteins. in Methods in Molecular Biology. vol. 1489, Methods in Molecular Biology, vol. 1489, Humana Press Inc., pp. 139-143. https://doi.org/10.1007/978-1-4939-6430-7_14
Kawaki H, Kubota S, Takigawa M. Analysis of signaling pathways activated by CCN proteins. In Methods in Molecular Biology. Vol. 1489. Humana Press Inc. 2017. p. 139-143. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6430-7_14
Kawaki, Harumi ; Kubota, Satoshi ; Takigawa, Masaharu. / Analysis of signaling pathways activated by CCN proteins. Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. pp. 139-143 (Methods in Molecular Biology).
@inbook{4c16f3b0658c42c1be8166f596a9316b,
title = "Analysis of signaling pathways activated by CCN proteins",
abstract = "CCN family proteins activate multiple intracellular phosphorylated kinase cascades to yield the multiple physiological functions of a variety of target cells. In this chapter, we describe our protocol examining the effects of these proteins on signal transduction pathways, especially mitogen-activated protein kinase cascades, activated by CCN member proteins, which examinations have been carried out mainly by using Western blotting methodologies.",
keywords = "CCN signaling, ERK1/2 (p44/42 MAPK), JNK, MAPK pathway, p38 MAPK, PI3K-PKB/Akt pathway, Western blotting",
author = "Harumi Kawaki and Satoshi Kubota and Masaharu Takigawa",
year = "2017",
doi = "10.1007/978-1-4939-6430-7_14",
language = "English",
volume = "1489",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "139--143",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Analysis of signaling pathways activated by CCN proteins

AU - Kawaki, Harumi

AU - Kubota, Satoshi

AU - Takigawa, Masaharu

PY - 2017

Y1 - 2017

N2 - CCN family proteins activate multiple intracellular phosphorylated kinase cascades to yield the multiple physiological functions of a variety of target cells. In this chapter, we describe our protocol examining the effects of these proteins on signal transduction pathways, especially mitogen-activated protein kinase cascades, activated by CCN member proteins, which examinations have been carried out mainly by using Western blotting methodologies.

AB - CCN family proteins activate multiple intracellular phosphorylated kinase cascades to yield the multiple physiological functions of a variety of target cells. In this chapter, we describe our protocol examining the effects of these proteins on signal transduction pathways, especially mitogen-activated protein kinase cascades, activated by CCN member proteins, which examinations have been carried out mainly by using Western blotting methodologies.

KW - CCN signaling

KW - ERK1/2 (p44/42 MAPK)

KW - JNK

KW - MAPK pathway

KW - p38 MAPK

KW - PI3K-PKB/Akt pathway

KW - Western blotting

UR - http://www.scopus.com/inward/record.url?scp=84991735066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991735066&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6430-7_14

DO - 10.1007/978-1-4939-6430-7_14

M3 - Chapter

C2 - 27734373

AN - SCOPUS:84991735066

VL - 1489

T3 - Methods in Molecular Biology

SP - 139

EP - 143

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -