TY - JOUR
T1 - Analysis of sensitization to carboxymethylcellulose
T2 - Identification of high risk group using ELISA and histamine release experiment
AU - Mori, S.
AU - Muroi, N.
AU - Ono, S.
AU - Takahashi, H. K.
AU - Nishibori, M.
N1 - Funding Information:
Acknowledgments. This work was supported by grants from Suzuken Memorial Foundation, Kurozumi Medical Research Foundation, Mishi-makaiun Memorial Foundation, and Protein 3000 Project, Japan.
PY - 2004/4
Y1 - 2004/4
N2 - Objective and Design: Carboxymethylcellulose (CMC) has been considered to be inert and is commonly used as an additive in medicines, foods and cosmetics. However, we experienced a patient who developed an anaphylactic reaction to CMC after an upper gastrointestinal examination using a barium meal containing CMC. Therefore, we examined the incidence of sensitization by CMC in healthy subjects, and categorized the high risk group prone to developing anaphylactic response to CMC. Methods: An ELISA for detecting CMC-specific IgE antibody was developed using serum from the patient as a positive control. In the ten subjects exhibiting high anti-CMC IgE among 387 normal populations, histamine release from isolated leukocytes was performed. Results: Five of ten subjects with a high IgE titer showed a significant CMC-induced histamine release from leukocyte preparations in vitro as observed in the patient, and were classified as high risk group. There was a correlation between sensitization by CMC and that by Japanese cedar pollen. The incidence of sensitization in females was 2.4 fold higher than that in males. Conclusions: The combination of ELISA and histamine release experiment made it possible to identify the high risk group for developing anaphylactic response. The administration of high dose CMC as a suspending agent in barium sulfate or injectable corticosteroids to this group should be avoided to prevent anaphylactic reactions in the clinic.
AB - Objective and Design: Carboxymethylcellulose (CMC) has been considered to be inert and is commonly used as an additive in medicines, foods and cosmetics. However, we experienced a patient who developed an anaphylactic reaction to CMC after an upper gastrointestinal examination using a barium meal containing CMC. Therefore, we examined the incidence of sensitization by CMC in healthy subjects, and categorized the high risk group prone to developing anaphylactic response to CMC. Methods: An ELISA for detecting CMC-specific IgE antibody was developed using serum from the patient as a positive control. In the ten subjects exhibiting high anti-CMC IgE among 387 normal populations, histamine release from isolated leukocytes was performed. Results: Five of ten subjects with a high IgE titer showed a significant CMC-induced histamine release from leukocyte preparations in vitro as observed in the patient, and were classified as high risk group. There was a correlation between sensitization by CMC and that by Japanese cedar pollen. The incidence of sensitization in females was 2.4 fold higher than that in males. Conclusions: The combination of ELISA and histamine release experiment made it possible to identify the high risk group for developing anaphylactic response. The administration of high dose CMC as a suspending agent in barium sulfate or injectable corticosteroids to this group should be avoided to prevent anaphylactic reactions in the clinic.
KW - Anaphylaxis
KW - Carboxymethylcellulose
KW - ELISA
KW - High risk
KW - Histamine release
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U2 - 10.1007/s00011-003-1238-9
DO - 10.1007/s00011-003-1238-9
M3 - Article
C2 - 15060723
AN - SCOPUS:1942518302
VL - 53
SP - 164
EP - 169
JO - Inflammation Research
JF - Inflammation Research
SN - 1023-3830
IS - 4
ER -