Analysis of seawaters for the recovery of culturable Vibrio parahaemolyticus and some other vibrios

J. M. Alam, K. I. Tomochika, S. I. Miyoshi, S. Shinoda

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

We investigated the recovery of dormant and injured cells along with the normally culturable cells of Vibrio species with special emphasis on V. parahaemolyticus using both selective and non-selective media at moderate (20 C) and standard (37 C) culture temperatures from a bay water environment. Culture temperatures (20 or 37 C) did not affect the recovery of V. parahaemolyticus but did for other vibrios. We observed similar seasonality of V. parahaemolyticus as in most other environmental studies. V. parahaemolyticus and other Vibrio species were recovered in higher numbers by a replica plating method compared to most probable number (MPN) and direct TCBS (thiosulfate citrate bile-salt sucrose) agar counts. Even with the replica plating method, however, vibrios number goes down to a minimum level and V. parahaemolyticus was undetectable during the cool temperature period of the year, although total bacterial cells and CFU on nutrient agar (with 2% NaCl) did not vary so much during the study period.

Original languageEnglish
Pages (from-to)393-397
Number of pages5
JournalMICROBIOLOGY and IMMUNOLOGY
Volume45
Issue number5
DOIs
Publication statusPublished - Jan 1 2001

Keywords

  • Ecology of vibrios
  • Seawater analysis
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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