Analysis of RNA-seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus

Tetsuya Bando, Yoshiyasu Ishimaru, Takuro Kida, Yoshimasa Hamada, Yuji Matsuoka, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji, Taro Mito

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In the cricket Gryllus bimaculatus, missing distal parts of the amputated leg are regenerated from the blastema, a population of dedifferentiated proliferating cells that forms at the distal tip of the leg stump. To identify molecules involved in blastema formation, comparative transcriptome analysis was performed between regenerating and normal unamputated legs. Components of JAK/STAT signalling were upregulated more than twofold in regenerating legs. To verify their involvement, Gryllus homologues of the interleukin receptor Domeless (Gb'dome), the Janus kinase Hopscotch (Gb'hop) and the transcription factor STAT (Gb'Stat) were cloned, and RNAi was performed against these genes. Gb'domeRNAi, Gb'hopRNAi and Gb'StatRNAi crickets showed defects in leg regeneration. Blastema expression of Gb'cyclinE was decreased in the Gb'StatRNAi cricket compared with that in the control. Hyperproliferation of blastema cells caused by Gb'fatRNAi or Gb'wartsRNAi was suppressed by RNAi against Gb'Stat. The results suggest that JAK/STAT signalling regulates blastema cell proliferation during leg regeneration.

Original languageEnglish
Pages (from-to)959-964
Number of pages6
JournalDevelopment
Volume140
Issue number5
DOIs
Publication statusPublished - 2013

Fingerprint

Gryllidae
Regeneration
Leg
RNA
RNA Interference
Interleukin Receptors
STAT Transcription Factors
Janus Kinases
Humulus
Gene Expression Profiling
Cell Proliferation
Population
Genes

Keywords

  • Blastema
  • Gryllus bimaculatus
  • JAK/STAT signalling
  • Next-generation sequencer
  • Regeneration
  • Transcriptome

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Analysis of RNA-seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus. / Bando, Tetsuya; Ishimaru, Yoshiyasu; Kida, Takuro; Hamada, Yoshimasa; Matsuoka, Yuji; Nakamura, Taro; Ohuchi, Hideyo; Noji, Sumihare; Mito, Taro.

In: Development, Vol. 140, No. 5, 2013, p. 959-964.

Research output: Contribution to journalArticle

Bando, Tetsuya ; Ishimaru, Yoshiyasu ; Kida, Takuro ; Hamada, Yoshimasa ; Matsuoka, Yuji ; Nakamura, Taro ; Ohuchi, Hideyo ; Noji, Sumihare ; Mito, Taro. / Analysis of RNA-seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus. In: Development. 2013 ; Vol. 140, No. 5. pp. 959-964.
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