Abstract
Nicotiana benthamiana was transformed with the NSs gene sequence of Tomato spotted wilt virus (TSWV). When a non-silenced line showing high levels of the transgene expression was inoculated with Potato virus X (PVX) engineered to contain the NSs gene sequences, reduced transgene mRNA accumulation was observed. RNA silencing was the underlying mechanism for the reduction. Previously, non-transgenic N. benthamiana infected with PVX engineered to contain the nucleocapsid protein (N) gene of TSWV was shown to be resistant to TSWV infection [Plant Sci. 164 (2003) 717]. In the present study, interactions between PVX engineered to contain the NSs gene sequences and TSWV were examined. Plants infected with PVX containing the NSs gene sequences showed a partial virus resistance to TSWV. However, the degree of the resistance was apparently lower than that observed in plants infected with PVX engineered to contain the N gene. Similar low levels of the resistance to TSWV was observed in plants infected with PVX engineered to contain the NSm gene of TSWV. These results suggest that RNA-mediated virus resistance conferred by the NSs and NSm gene sequences of TSWV was less effective to TSWV. Furthermore, it was also suggested that RNA-mediated virus resistance was affected by viral genes or sequences rather than the amount of nucleotide sequence homology.
Original language | English |
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Pages (from-to) | 771-778 |
Number of pages | 8 |
Journal | Plant Science |
Volume | 166 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2004 |
Keywords
- Cross-protection
- Nicotiana benthamiana
- RNA silencing
- TSWV
- Virus resistance
ASJC Scopus subject areas
- Genetics
- Agronomy and Crop Science
- Plant Science