Analysis of proteins encoded in the bipartite genome of a new type of parvo-like virus isolated from silkworm - Structural protein with DNA polymerase motif

Tohru Hayakawa, Katsura Kojima, Kiichirou Nonaka, Masao Nakagaki, Ken Sahara, Shin Ichiro Asano, Toshihiko Iizuka, Hisanori Bando

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Bombyx mori densonucleosis virus type 2 (BmDNV-2) is a small, spherical virus containing two complementary single-stranded linear DNA molecules (VD1, VD2). BmDNV-2 is a new type of virus with a unique, yet unspecified replication mechanism which is different from that of parvoviruses (Bando, H., Choi, H., Ito, Y., Nakagaki, M., Kawase, S., 1992. Structural analysis on the single-stranded genomic DNAs of the virus newly isolated from silkworm: the DNA molecules share a common terminal sequence, Arch. Virol. 124, 187- 193; Bando, H., Hayakawa, T., Asano, S., Sahara, K., Nakagaki, M., Iizuka, T., 1995. Analysis of the genetic information of a DNA segment of a new virus from silkworm, Arch. Virol., 140, 1147-1155; Hayakawa, T., Asano, S., Sahara, K., Iizuka, T., Bando, H., 1997. Detection of replicative intermediate with closed terminus of Bombyx densonucleosis virus. Arch. Virol. 142, 1-7). Recent analyses on the genomic information of BmDNV-2 identified open reading frames which code for three tentative nonstructural proteins and four (VP1 to 4) of the six known structural proteins (Bando, H., Hayakawa, T., Asano, S., Sahara, K., Nakagaki, M., Iizuka, T., 1995. Analysis of the genetic information of a DNA segment of a new virus from silkworm, Arch. Virol., 140, 1147-1155; Nakagaki et al., in preparation). In this report we demonstrate that the two largest ORFs, VD1-ORF1 and VD2-ORF1, code for the two remaining structural proteins. In addition, computer-assisted analysis revealed that the structural protein encoded in VD1-ORF1 contains sequences conserved among various DNA polymerases, and showed an evolutionary relationship with the DNA polymerases involved in protein-primed replication. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalVirus research
Volume66
Issue number1
DOIs
Publication statusPublished - Jan 1 2000
Externally publishedYes

Keywords

  • DNA polymerase
  • Parvo-like virus
  • Structural proteins

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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