Analysis of progranulin-mediated Akt and MAPK activation

Shi Qiong Xu, Simone Buraschi, Ryuta Tanimoto, Manuela Stefanello, Antonino Belfiore, Renato V. Iozzo, Andrea Morrione

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Progranulin has emerged in recent years as an important regulator of various biological functions including cell proliferation, wound healing, motility, and protection from apoptosis. Progranulin is also critical for transformation as established in several cancer models. Progranulin biological responses elicit through the activation of the Akt and MAPK pathways, which are critical for progranulin downstream signaling. In this chapter various experimental approaches aiming at detecting progranulin-mediated Akt and MAPK activation will be discussed.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages121-130
Number of pages10
DOIs
Publication statusPublished - Jan 1 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1806
ISSN (Print)1064-3745

    Fingerprint

Keywords

  • Akt
  • Confocal analysis
  • Electrophoresis
  • Immunofluorescence
  • MAP kinase
  • Nitrocellulose membrane
  • PathScan
  • Progranulin
  • SDS PAGE

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Xu, S. Q., Buraschi, S., Tanimoto, R., Stefanello, M., Belfiore, A., Iozzo, R. V., & Morrione, A. (2018). Analysis of progranulin-mediated Akt and MAPK activation. In Methods in Molecular Biology (pp. 121-130). (Methods in Molecular Biology; Vol. 1806). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8559-3_9